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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF7217 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal RNF168 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects recombinant mouse RNF168 and recombinant human RNF168 in direct ELISAs and Western blots.
- Reactivity
- Human, Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation.
Guturi KKN, Bohgaki M, Bohgaki T, Srikumar T, Ng D, Kumareswaran R, El Ghamrasni S, Jeon J, Patel P, Eldin MS, Bristow R, Cheung P, Stewart GS, Raught B, Hakem A, Hakem R
Nature communications 2016 Aug 25;7:12638
Nature communications 2016 Aug 25;7:12638
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse RNF168 by Western Blot. Western blot shows lysates of mouse brain (cortex) tissue and mouse brain (thalamus/hypothalamus) tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse RNF168 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7217) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RNF168 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
