Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00165918-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00165918-M01, RRID:AB_2180102
- Product name
- Mouse Monoclonal RNF168 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RNF168 (3E1)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination.
Monoubiquitinated γ-H2AX: Abundant product and specific biomarker for non-apoptotic DNA double-strand breaks.
Sharma A, Alswillah T, Singh K, Chatterjee P, Willard B, Venere M, Summers MK, Almasan A
Autophagy 2018;14(11):1976-1990
Autophagy 2018;14(11):1976-1990
Monoubiquitinated γ-H2AX: Abundant product and specific biomarker for non-apoptotic DNA double-strand breaks.
Luczak MW, Zhitkovich A
Toxicology and applied pharmacology 2018 Sep 15;355:238-246
Toxicology and applied pharmacology 2018 Sep 15;355:238-246
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: RNF168 Antibody (3E1) [H00165918-M01] - RNF168 monoclonal antibody (M01), clone 3E1 Analysis of RNF168 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: RNF168 Antibody (3E1) [H00165918-M01] - Detection limit for recombinant GST tagged RNF168 is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: RNF168 Antibody (3E1) [H00165918-M01] - Analysis of monoclonal antibody to RNF168 on HepG2 cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: RNF168 Antibody (3E1) [H00165918-M01] - Analysis of monoclonal antibody to RNF168 on formalin-fixed paraffin-embedded human prostate cancer. Antibody concentration 1.5 ug/ml.