Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- 21393-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RNF168 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: NTGDIQKYL TPKSQFGSAS HSEAVQEVRK DSVSKDIDSS DRKSPTGQDT EIEDMPTLSP QISLGVGEQG ADSSIESPMP WLCACGAEWY HEGNVKTRPS NHGKELCVLS HERPKTRVPY SKETAVMPCG RTESGCAPTS GVTQTNGNNT GETENEESCL LISKEISKRK NQESSFEAVK DPCFSAKRRK VSPESSPDQE ETEINFTQKL IDLEHLLFER HKQEEQDRLL ALQLQKEVDK EQMVPNRQKG SPDEYHLRAT SSPPDKVLNG QRKNPKDGNF KRQTHTKHPT PERGSRDKNR QVSLKMQLKQ SVNRRKMPNS TRDHCKVSKS AHSLQPSISQ KSVFQMFQRC TK (221-571 aa encoded by BC033791)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.49 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HepG2 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RNF168 antibody 21393-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-RNF168 (IP:21393-1-AP, 4ug; Detection:21393-1-AP 1:500) with HepG2 cells lysate 2400ug.