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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-51312 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPL35A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The antibody detects endogenous levels of total RPL35A protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPL35A was performed by loading Lane 1-4: PC-3, 231, 293T and Hela cell lysates (40µg) on to a 12% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a RPL35A antibody (Product # PA5-51312) at a 1/200 dilution for 1 minute, followed by Goat anti rabbit IgG at 1/8000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPL35A in paraffin-embedded Human brain tissue. Samples were probed using a RPL35A polyclonal antibody (Product # PA5-51312) at a dilution of 1/20.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPL35A in paraffin embedded Human Esophagus tissue using RPL35A Polyclonal Antibody (Product # PA5-51312) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of RPL35A in paraffin embedded Human Thyroid tissue using RPL35A Polyclonal Antibody (Product # PA5-51312) at a 1:200 dilution.
