LS-C185542

antibody from LSBio

Targeting: PRKAA2 AMPK, AMPKa2, PRKAA

Western blot

Antibody data

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Validation data

Product number: LS-C185542 - Provider product page
Provider: LSBio
Product name: PRKAA2 / AMPK Alpha 2 Antibody (aa29-510) LS-C185542
Antibody type: Polyclonal
Description: Immunoaffinity purified
Reactivity: Human
Host: Rabbit
Isotype: IgG
Storage: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

PRKAA2 protein structure - LS-C185542 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 ug of AMPK alpha 2 antibody 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 ug of AMPK alpha 2 antibody 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.

Supportive validation

Submitted by: LSBio (provider)
Main image
Experimental details: AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample:1000 ug 293T whole cell lysate/extract A. 30 ug 293T whole cell lysate/extract B. Control with 2.5 ug of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 ug of AMPK alpha 2 antibody 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (anti-rabbit IgG (HRP) -01) was used as a secondary reagent.