18167-1-AP

antibody from Proteintech Group

Targeting: PRKAA2 AMPK, AMPKa2, PRKAA

Provider product page for 18167-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [24]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]

Submit

Product number: 18167-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#18167-1-AP, RRID:AB_10695046
Product name: AMPK Alpha 2 antibody
Antibody type: Polyclonal
Description: KD/KO validated AMPK Alpha 2 antibody (Cat. #18167-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

PRKAA2 protein structure - 18167-1-AP shown in red.

Submitted references UBE2O promotes hepatocellular carcinoma cell proliferation and invasion by regulating the AMPKα2/mTOR pathway.

Shi Z, Liu R, Lu Q, Zeng Z, Liu Y, Zhao J, Liu X, Li L, Huang H, Yao Y, Huang D, Xu Q
International journal of medical sciences 2021;18(16):3749-3758

TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.

Zhou C, Liang Y, Zhou L, Yan Y, Liu N, Zhang R, Huang Y, Wang M, Tang Y, Ali DW, Wang Y, Michalak M, Chen XZ, Tang J
Autophagy 2021 Oct;17(10):3175-3195

SIRT3 affects mitochondrial metabolic reprogramming via the AMPK-PGC-1α axis in the development of benign prostatic hyperplasia.

Li Y, Wang Q, Li J, Shi B, Liu Y, Wang P
The Prostate 2021 Nov;81(15):1135-1148

Naringin induces skeletal muscle fiber type transformation via AMPK/PGC-1α signaling pathway in mice and C2C12 myotubes.

Xue Y, Huang Z, Chen X, Jia G, Zhao H, Liu G
Nutrition research (New York, N.Y.) 2021 Aug;92:99-108

Metformin Mitigates Cartilage Degradation by Activating AMPK/SIRT1-Mediated Autophagy in a Mouse Osteoarthritis Model.

Wang C, Yao Z, Zhang Y, Yang Y, Liu J, Shi Y, Zhang C
Frontiers in pharmacology 2020;11:1114

Si-Miao-Yong-An Decoction attenuates isoprenaline-induced myocardial fibrosis in AMPK-driven Akt/mTOR and TGF-β/SMAD3 pathways.

Zhao Y, Sun D, Chen Y, Zhan K, Meng Q, Zhang X, Zhu L, Yao X
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2020 Oct;130:110522

Normothermic Machine Perfusion Combined with Bone Marrow Mesenchymal Stem Cells Improves the Oxidative Stress Response and Mitochondrial Function in Rat Donation After Circulatory Death Livers.

Yang L, Cao H, Sun D, Lin L, Zheng WP, Shen ZY, Song HL
Stem cells and development 2020 Jul 1;29(13):835-852

UBE2O promotes the proliferation, EMT and stemness properties of breast cancer cells through the UBE2O/AMPKα2/mTORC1-MYC positive feedback loop.

Liu X, Ma F, Liu C, Zhu K, Li W, Xu Y, Li G, Niu Z, Liu J, Chen D, Li Z, Fu Y, Qian C
Cell death & disease 2020 Jan 6;11(1):10

Whether AICAR in Pregnancy or Lactation Prevents Hypertension Programmed by High Saturated Fat Diet: A Pilot Study.

Tsai WL, Hsu CN, Tain YL
Nutrients 2020 Feb 11;12(2)

Activation of AMPK suppresses S1P-induced airway smooth muscle cells proliferation and its potential mechanisms.

Pan Y, Liu L, Zhang Q, Shi W, Feng W, Wang J, Wang Q, Li S, Li M
Molecular immunology 2020 Dec;128:106-115

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Fu Y, Sun Y, Wang M, Hou Y, Huang W, Zhou D, Wang Z, Yang S, Tang W, Zhen J, Li Y, Wang X, Liu M, Zhang Y, Wang B, Liu G, Yu X, Sun J, Zhang C, Yi F
Cell metabolism 2020 Dec 1;32(6):1052-1062.e8

Dual-specificity phosphatase 29 is induced during neurogenic skeletal muscle atrophy and attenuates glucocorticoid receptor activity in muscle cell culture.

Cooper LM, West RC, Hayes CS, Waddell DS
American journal of physiology. Cell physiology 2020 Aug 1;319(2):C441-C454

Hepatitis C virus nonstructural protein 5A perturbs lipid metabolism by modulating AMPK/SREBP-1c signaling.

Meng Z, Liu Q, Sun F, Qiao L
Lipids in health and disease 2019 Nov 4;18(1):191

Activation of AMPK inhibits TGF-β1-induced airway smooth muscle cells proliferation and its potential mechanisms.

Pan Y, Liu L, Li S, Wang K, Ke R, Shi W, Wang J, Yan X, Zhang Q, Wang Q, Chai L, Xie X, Li M
Scientific reports 2018 Feb 26;8(1):3624

Apelin‑13 ameliorates metabolic and cardiovascular disorders in a rat model of type 2 diabetes with a high‑fat diet.

Li M, Fang H, Hu J
Molecular medicine reports 2018 Dec;18(6):5784-5790

Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway.

Ji J, Xue TF, Guo XD, Yang J, Guo RB, Wang J, Huang JY, Zhao XJ, Sun XL
Aging cell 2018 Aug;17(4):e12774

Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy and Inhibiting Excessive Astrocytic Apoptosis.

Zhao Z, Zhang L, Guo XD, Cao LL, Xue TF, Zhao XJ, Yang DD, Yang J, Ji J, Huang JY, Sun XL
Frontiers in molecular neuroscience 2017;10:293

PD149163 induces hypothermia to protect against brain injury in acute cerebral ischemic rats.

Xue TF, Ding X, Ji J, Yan H, Huang JY, Guo XD, Yang J, Sun XL
Journal of pharmacological sciences 2017 Nov;135(3):105-113

AMP-activated protein kinase-mediated expression of heat shock protein beta 1 enhanced insulin sensitivity in the skeletal muscle.

Yuan H, Wang T, Niu Y, Liu X, Fu L
FEBS letters 2017 Jan;591(1):97-108

AMPK deficiency in chondrocytes accelerated the progression of instability-induced and ageing-associated osteoarthritis in adult mice.

Zhou S, Lu W, Chen L, Ge Q, Chen D, Xu Z, Shi D, Dai J, Li J, Ju H, Cao Y, Qin J, Chen S, Teng H, Jiang Q
Scientific reports 2017 Feb 22;7:43245

Extracellular pH regulates autophagy via the AMPK-ULK1 pathway in rat cardiomyocytes.

Zhao L, Cui L, Jiang X, Zhang J, Zhu M, Jia J, Zhang Q, Zhang J, Zhang D, Huang Y
FEBS letters 2016 Sep;590(18):3202-12

Activation of AMPK α2 inhibits airway smooth muscle cells proliferation.

Liu L, Pan Y, Song Y, Su X, Ke R, Yang L, Gao L, Li M
European journal of pharmacology 2016 Nov 15;791:235-243

Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27(Kip1) Signaling Pathway.

Ke R, Liu L, Zhu Y, Li S, Xie X, Li F, Song Y, Yang L, Gao L, Li M
International journal of molecular sciences 2016 May 31;17(6)

Glutaredoxins concomitant with optimal ROS activate AMPK through S-glutathionylation to improve glucose metabolism in type 2 diabetes.

Dong K, Wu M, Liu X, Huang Y, Zhang D, Wang Y, Yan LJ, Shi D
Free radical biology & medicine 2016 Dec;101:334-347

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP(PRKAA2 antibody) at dilution of 1:500
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of MCF-7 cells, using PRKAA2 antibody 18167-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line