GTX103487

Wang H, Shen X, Tian G, Shi X, Huang W, Wu Y, Sun L, Peng C, Liu S, Huang Y, Chen X, Zhang F, Chen Y, Ding W, Lu Z
Free radical biology & medicine 2018 Jun;121:202-214

AMP-activated kinase α2 deficiency protects mice from denervation-induced skeletal muscle atrophy.

Guo Y, Meng J, Tang Y, Wang T, Wei B, Feng R, Gong B, Wang H, Ji G, Lu Z
Archives of biochemistry and biophysics 2016 Jun 15;600:56-60

AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid.

Zhou X, Wu W, Chen J, Wang X, Wang Y
Nutrition & metabolism 2015;12:10

Selenium-enriched exopolysaccharides improve skeletal muscle glucose uptake of diabetic KKAy mice via AMPK pathway.

Zhou X, Chen J, Wang F, Yang H, Yang R, Wang X, Wang Y
Journal of physiology and biochemistry 2014 Jun;70(2):547-54

Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.

Lee YZ, Yang CW, Chang HY, Hsu HY, Chen IS, Chang HS, Lee CH, Lee JC, Kumar CR, Qiu YQ, Chao YS, Lee SJ
Oncotarget 2014 Aug 15;5(15):6087-101

Trichostatin A and sirtinol suppressed survivin expression through AMPK and p38MAPK in HT29 colon cancer cells.

Hsu YF, Sheu JR, Lin CH, Yang DS, Hsiao G, Ou G, Chiu PT, Huang YH, Kuo WH, Hsu MJ
Biochimica et biophysica acta 2012 Feb;1820(2):104-15

Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK.

Lin YY, Kiihl S, Suhail Y, Liu SY, Chou YH, Kuang Z, Lu JY, Khor CN, Lin CL, Bader JS, Irizarry R, Boeke JD
Nature 2012 Feb 8;482(7384):251-5

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Supportive validation

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Main image
Experimental details: Sample (30 ug of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX103487 diluted at 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: AMPK alpha 2 antibody detects PRKAA2 protein by Western blot analysis.A. 30 ?g A431 whole cell lysate/extract B. 30 ?g HeLa whole cell lysate/extract C. 30 ?g A375 whole cell lysate/extract7.5 % SDS-PAGEAMPK alpha 2 antibody (GTX103487) dilution: 1:1000

Supportive validation

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Main image
Experimental details: Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AMPK alpha 2 antibody (GTX103487) diluted at 1:1000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AMPK alpha 2 protein stained by AMPK alpha 2 antibody (GTX103487) diluted at 1:400.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 £gg 293T whole cell lysate/extract A. 30 £gg 293T whole cell lysate/extract B. Control with 2.5 £gg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 £gg of AMPK alpha 2 antibody (GTX103487) 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody (GTX103487) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytosol on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. AMPK alpha 2 antibody (GTX103487) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. AMPK alpha 2 antibody (GTX103487) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: AMPK alpha 2 antibody detects AMPK alpha 2 protein at cytoplasm in rat muscle by immunohistochemical analysis. Sample: Paraffin-embedded rat muscle. AMPK alpha 2 antibody (GTX103487) diluted at 1:500.