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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00005563-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005563-M02, RRID:AB_608274
- Product name
- Mouse Monoclonal AMPK alpha 2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PRKAA2 - protein kinase, AMP-activated, alpha 2 catalytic subunit
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of PRKAA2 over-expressed 293 cell line, cotransfected with PRKAA2 Validated Chimera RNAi ( Cat # H00005563-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PRKAA2 monoclonal antibody (M02) clone 1G8 (Cat # H00005563-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of PRKAA2 expression in transfected 293T cell line by PRKAA2 monoclonal antibody (M02), clone 1G8.Lane 1: PRKAA2 transfected lysate(62.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Detection limit for recombinant GST tagged PRKAA2 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: AMPK alpha 2 Antibody (1G8) [H00005563-M02] - Analysis of monoclonal antibody to PRKAA2 on HeLa cell . Antibody concentration 10 ug/ml.
