PAB28533

antibody from Abnova Corporation

Targeting: GPKOW GPATC5, GPATCH5, Mos2, Spp2, T54

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28533

Provider

Abnova Corporation

Product name

GPKOW polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GPKOW.

Antigen sequence

MPRPDEEQEKDKEDQPQGLVPGGAVVVLSGPHRGL
YGKVEGLDPDNVRAMVRLAVGSRVVTVSEYYLRPV
SQQEFDKNTLDLRQQNGTASSRKTLWNQELYIQQD
NSERKRKHLPD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: A-431, Lane 4: Liver, Lane 5: Tonsil with GPKOW polyclonal antibody (Cat # PAB28533) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with GPKOW polyclonal antibody (Cat # PAB28533) at 1-4 ug/ml shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with GPKOW polyclonal antibody (Cat # PAB28533) shows strong nuclear positivity in exocrine glandular cells and islet cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)