PAB22509

antibody from Abnova Corporation

Targeting: ZC3H15 LEREPO4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB22509

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB22509, RRID:AB_10965948

Product name

ZC3H15 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ZC3H15.

Antigen sequence

IVCKHFLEAIENNKYGWFWVCPGGGDICMYRHALP
PGFVLKKDKKKEEKEDEISLEDLIERERSALGPNV
TKITL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ZC3H15 polyclonal antibody (Cat # PAB22509) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with ZC3H15 polyclonal antibody (Cat # PAB22509) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with ZC3H15 polyclonal antibody (Cat # PAB22509) strong cytoplasmic positivity in glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)