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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 619402 - Provider product page
- Provider
- BioLegend
- Product name
- Purified anti-Histone H2A
- Antibody type
- Polyclonal
- Antigen
- Peptide mixture containing amino acid resides 1-5 and 81-96 of histone H2A
- Description
- Histone H2A is a 14 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation. Histone H2A can be modified by phosphorylation and acetylation; phosphorylation of H2A correlates with mitotic chromosome condensation. Histone H2A interacts with Histones H3B, H3, and H4 as well as HIV-1 Tat peptides. The Poly6194 antibody has been shown to be useful for the Western blotting of human histone 2A.
- Reactivity
- Human, Zebrafish
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Jurkat cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-Histone H2A polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated
