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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-14245 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- COL5A2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Increased Collagen Type V α2 (COL5A2) in Colorectal Cancer is Associated with Poor Prognosis and Tumor Progression.
Wang J, Jiang YH, Yang PY, Liu F
OncoTargets and therapy 2021;14:2991-3002
OncoTargets and therapy 2021;14:2991-3002
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a COL5A2 polyclonal antibody (Product # PA5-14245) in mouse lung tissue lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of NCI-H460 cells using a COL5A2 polyclonal antibody (Product # PA5-14245) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Expression of COL5A2 protein in colon cancer tissues. Immunohistochemistry (IHC) analysis was performed using two commercial tissue microarrays (TMAs) containing a total of 180 cases. ( A ) The image shows the negative or low expression of COL5A2 in normal colonic mucosa tissue. The bar represents 500 um. The boxed area is enlarged to the right side. ( B ) Low and ( C ) high expression of COL5A2 in CRC tissue. ( D ) A statistic of COL5A2 expression between the normal and cancer tissues based on the scanned staining intensity values. Abbreviations: N_full, normal tissue (full tissue spot on the TMA); C_full, cancer tissue (full tissue spot on the TMA); N_str, normal tissue, stromal part; C_str, cancer tissue, stromal part; N_epi, normal tissue, epithelial part; C_epi, cancer tissue, epithelial part.
