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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Other assay [4]
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- Product number
- AHR0271 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INSR beta Monoclonal Antibody (CT-3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- Staining of formalin-fixed paraffin embedded tissue sections requires boiling the sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- CT-3
- Vial size
- 500 µL
- Concentration
- 0.05 mg/mL
- Storage
- 4° C
Submitted references Modulation of olfactory-driven behavior by metabolic signals: role of the piriform cortex.
Suppression of insulin feedback enhances the efficacy of PI3K inhibitors.
High expression of insulin receptor on tumour-associated blood vessels in invasive bladder cancer predicts poor overall and progression-free survival.
A persistent increase in insulin-stimulated glucose uptake by both fast-twitch and slow-twitch skeletal muscles after a single exercise session by old rats.
A physiological increase of insulin in the olfactory bulb decreases detection of a learned aversive odor and abolishes food odor-induced sniffing behavior in rats.
Preventing the calorie restriction-induced increase in insulin-stimulated Akt2 phosphorylation eliminates calorie restriction's effect on glucose uptake in skeletal muscle.
Calorie restriction enhances insulin-stimulated glucose uptake and Akt phosphorylation in both fast-twitch and slow-twitch skeletal muscle of 24-month-old rats.
Generation of intracellular domain of insulin receptor tyrosine kinase by gamma-secretase.
In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.
In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.
Al Koborssy D, Palouzier-Paulignan B, Canova V, Thevenet M, Fadool DA, Julliard AK
Brain structure & function 2019 Jan;224(1):315-336
Brain structure & function 2019 Jan;224(1):315-336
Suppression of insulin feedback enhances the efficacy of PI3K inhibitors.
Hopkins BD, Pauli C, Du X, Wang DG, Li X, Wu D, Amadiume SC, Goncalves MD, Hodakoski C, Lundquist MR, Bareja R, Ma Y, Harris EM, Sboner A, Beltran H, Rubin MA, Mukherjee S, Cantley LC
Nature 2018 Aug;560(7719):499-503
Nature 2018 Aug;560(7719):499-503
High expression of insulin receptor on tumour-associated blood vessels in invasive bladder cancer predicts poor overall and progression-free survival.
Roudnicky F, Dieterich LC, Poyet C, Buser L, Wild P, Tang D, Camenzind P, Ho CH, Otto VI, Detmar M
The Journal of pathology 2017 Jun;242(2):193-205
The Journal of pathology 2017 Jun;242(2):193-205
A persistent increase in insulin-stimulated glucose uptake by both fast-twitch and slow-twitch skeletal muscles after a single exercise session by old rats.
Xiao Y, Sharma N, Arias EB, Castorena CM, Cartee GD
Age (Dordrecht, Netherlands) 2013 Jun;35(3):573-82
Age (Dordrecht, Netherlands) 2013 Jun;35(3):573-82
A physiological increase of insulin in the olfactory bulb decreases detection of a learned aversive odor and abolishes food odor-induced sniffing behavior in rats.
Aimé P, Hegoburu C, Jaillard T, Degletagne C, Garcia S, Messaoudi B, Thevenet M, Lorsignol A, Duchamp C, Mouly AM, Julliard AK
PloS one 2012;7(12):e51227
PloS one 2012;7(12):e51227
Preventing the calorie restriction-induced increase in insulin-stimulated Akt2 phosphorylation eliminates calorie restriction's effect on glucose uptake in skeletal muscle.
Sharma N, Arias EB, Sequea DA, Cartee GD
Biochimica et biophysica acta 2012 Nov;1822(11):1735-40
Biochimica et biophysica acta 2012 Nov;1822(11):1735-40
Calorie restriction enhances insulin-stimulated glucose uptake and Akt phosphorylation in both fast-twitch and slow-twitch skeletal muscle of 24-month-old rats.
Sequea DA, Sharma N, Arias EB, Cartee GD
The journals of gerontology. Series A, Biological sciences and medical sciences 2012 Dec;67(12):1279-85
The journals of gerontology. Series A, Biological sciences and medical sciences 2012 Dec;67(12):1279-85
Generation of intracellular domain of insulin receptor tyrosine kinase by gamma-secretase.
Kasuga K, Kaneko H, Nishizawa M, Onodera O, Ikeuchi T
Biochemical and biophysical research communications 2007 Aug 17;360(1):90-6
Biochemical and biophysical research communications 2007 Aug 17;360(1):90-6
In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.
García-Echeverría C, Pearson MA, Marti A, Meyer T, Mestan J, Zimmermann J, Gao J, Brueggen J, Capraro HG, Cozens R, Evans DB, Fabbro D, Furet P, Porta DG, Liebetanz J, Martiny-Baron G, Ruetz S, Hofmann F
Cancer cell 2004 Mar;5(3):231-9
Cancer cell 2004 Mar;5(3):231-9
In vivo antitumor activity of NVP-AEW541-A novel, potent, and selective inhibitor of the IGF-IR kinase.
García-Echeverría C, Pearson MA, Marti A, Meyer T, Mestan J, Zimmermann J, Gao J, Brueggen J, Capraro HG, Cozens R, Evans DB, Fabbro D, Furet P, Porta DG, Liebetanz J, Martiny-Baron G, Ruetz S, Hofmann F
Cancer cell 2004 Mar;5(3):231-9
Cancer cell 2004 Mar;5(3):231-9
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of INSR/Insulin Receptor beta in HEPG2 cells using a INSR/Insulin Receptor beta monoclonal antibody (Product # AHR0271).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Insulin Receptor beta (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor beta monoclonal antibody (Product # AHR0271) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Insulin Receptor beta (green) showing staining in the cytoplasm of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor beta monoclonal antibody (Product # AHR0271) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Insulin Receptor beta (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with an Insulin Receptor beta monoclonal antibody (Product # AHR0271) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of INSR/Insulin Receptor beta in formalin-fixed, paraffin-embedded human placenta tissue using an INSR monoclonal antibody (Product # AHR0271). Detection was performed with a peroxidase-conjugate and AEC chromogen. Note cell membrane staining of trophoblasts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of INSR/Insulin Receptor beta in formalin-fixed, paraffin-embedded rat brain tissue using an INSR monoclonal antibody (Product # AHR0271). Detection was performed with a peroxidase-conjugate and AEC chromogen. Note staining of endothelial cells of capillaries.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
