18537-1-AP

antibody from Invitrogen Antibodies

Targeting: PRUNE1 DRES-17, H-PRUNE, HTCD37, PRUNE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

18537-1-AP

Provider

Invitrogen Antibodies

Product name

PRUNE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ILVDHHILS KSDTALEEAV AEVLDHRPIE PKHCPPCHVS VELVGSCATL VTERILQGAP EILDRQTAAL LHGTIILDCV NMDLKIGKAT PKDSKYVEKL EALFPDLPKR NDIFDSLQKA KFDVSGLTTE QMLRKDQKTI YRQGVKVAIS AIYMDLEAFL QRSNLLADLH AFCQAHSYDV LVAMTIFFNT HNEPVRQLAI FCPHVALQTT ICEVLERSHS PPLKLTPASS THPNLHAYLQ GNTQVSRKKL LPLLQEALSA YFDSMKIPSG QPETADVSRE QVDKELDRAS NSLISGLSQD EEDPPLPPTP MNSLVDECPL DQGLPKLSAE AVFEKCSQIS LSQSTTASLS KK (103-453 aa encoded by BC025304)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 18537-1-AP (PRUNE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 18537-1-AP (PRUNE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 18537-1-AP (PRUNE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PRUNE antibody 18537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PRUNE antibody 18537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PRUNE antibody 18537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using PRUNE antibody 18537-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer using 18537-1-AP (PRUNE antibody) at dilution of 1:100 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PRUNE (IP:18537-1-AP, 3ug; Detection:18537-1-AP 1:500) with HepG2 cells lysate 1720ug.