RQ8229

antibody from NSJ Bioreagents

Targeting: PRUNE1 DRES-17, H-PRUNE, HTCD37, PRUNE

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ8229

Provider

NSJ Bioreagents

Product name

PRUNE Antibody / Exopolyphosphatase PRUNE1

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the PRUNE antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) human 293T, 2) human Jurkat, 3) human HEL, 4) human MCF7, 5) rat brain, 6) mouse brain and 7) mouse RAW264.7 cell lysate with PRUNE antibody. Predicted molecular weight ~50 kDa (multiple isoforms) but commonly observed at 50-60 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of FFPE human U-2 OS cells with PRUNE antibody (red) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human 293T cells with PRUNE antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= PRUNE antibody.