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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- NBP1-04965 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-04965, RRID:AB_1520860
- Product name
- Rabbit Polyclonal eIF4G1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. eIF4G (phospho-Ser1232) detects endogenous levels of eIF4G only when phosphorylated at serine 1232.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mitotic phosphorylation of eukaryotic initiation factor 4G1 (eIF4G1) at Ser1232 by Cdk1:cyclin B inhibits eIF4A helicase complex binding with RNA.
Dynamic regulation of the translation initiation helicase complex by mitogenic signal transduction to eukaryotic translation initiation factor 4G.
Phosphorylation of eukaryotic translation initiation factor 4G1 (eIF4G1) by protein kinase C{alpha} regulates eIF4G1 binding to Mnk1.
Dobrikov MI, Shveygert M, Brown MC, Gromeier M
Molecular and cellular biology 2014 Feb;34(3):439-51
Molecular and cellular biology 2014 Feb;34(3):439-51
Dynamic regulation of the translation initiation helicase complex by mitogenic signal transduction to eukaryotic translation initiation factor 4G.
Dobrikov MI, Dobrikova EY, Gromeier M
Molecular and cellular biology 2013 Mar;33(5):937-46
Molecular and cellular biology 2013 Mar;33(5):937-46
Phosphorylation of eukaryotic translation initiation factor 4G1 (eIF4G1) by protein kinase C{alpha} regulates eIF4G1 binding to Mnk1.
Dobrikov M, Dobrikova E, Shveygert M, Gromeier M
Molecular and cellular biology 2011 Jul;31(14):2947-59
Molecular and cellular biology 2011 Jul;31(14):2947-59
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - Kinetics of eIF4G1 (s1232) phosphorylation in response to PKC/Ras/Erk activation by Sapintoxin D. Photo courtesy of a product review by the Gromeier Lab at Duke University.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - HT29 cells untreated or treated with Anisomycin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - Western blot analysis of extracts from 293 cell using eIF4G Antibody (Lane 1 and 2) and eIF4G (phospho-Ser1232) antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: eIF4G1 [p Ser1232] Antibody [NBP1-04965] - eIF4G1 [p Ser1232] Antibody Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (phospho- Ser1232) antibody
