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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [3]
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- Product number
- NBP1-89600 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89600, RRID:AB_11004771
- Product name
- Rabbit Polyclonal Cingulin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, rat Cingulin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Baicalin Protects Against Hypertension-Associated Intestinal Barrier Impairment in Part Through Enhanced Microbial Production of Short-Chain Fatty Acids.
Wu D, Ding L, Tang X, Wang W, Chen Y, Zhang T
Frontiers in pharmacology 2019;10:1271
Frontiers in pharmacology 2019;10:1271
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cingulin Antibody [NBP1-89600] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cingulin Antibody [NBP1-89600] - Analysis in human cell lines Caco-2 and HeLa using Anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cingulin Antibody [NBP1-89600] - Analysis using Anti-CGN antibody NBP1-89600 (A) shows similar pattern to independent antibody NBP1-89602 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining of human lymph node shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining of human small intestine shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Cingulin Antibody [NBP1-89600] - Staining in human small intestine and lymph node tissues using anti-CGN antibody. Corresponding CGN RNA-seq data are presented for the same tissues.
