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- Antibody Data
- Antigen structure
- References [30]
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- Product number
- MAB417-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-10 in sandwich ELISAs and Western blots. In sandwich ELISAs, less than 8% cross-reactivity with recombinant rat IL-10 and 1% cross-reactivity with recombinant cotton rat IL-10 is observed. In sandwich ELISAs, no cross-reactivity with recombinant human (rh) IL-10, recombinant mouse IL-10 R, rhIL-10 R, recombinant canine IL-10, recombinant feline IL-10, or recombinant porcine IL-10 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Antigen sequence
P18893- Isotype
- IgG
- Antibody clone number
- JES052A5
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Electroacupuncture at ST-36 Protects Interstitial Cells of Cajal via Sustaining Heme Oxygenase-1 Positive M2 Macrophages in the Stomach of Diabetic Mice.
BMSCs pre-treatment ameliorates inflammation-related tissue destruction in LPS-induced rat DIC model.
Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.
CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract.
PI3Kγ Activates Integrin α4 and Promotes Immune Suppressive Myeloid Cell Polarization during Tumor Progression.
Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3+CD49b-Foxp3- regulatory T cells.
A critical role for IRF5 in regulating allergic airway inflammation.
IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population.
Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response.
Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis.
Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2.
Delayed wound healing due to increased interleukin-10 expression in mice with lymphatic dysfunction.
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade.
Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis.
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis.
Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper.
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Sequential ELISA to profile multiple cytokines from small volumes.
Nonlymphoid peritoneal cells suppress the T cell response to Mls.
Selective macrophage suppression during sepsis.
Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
Tian L, Song S, Zhu B, Liu S
Oxidative medicine and cellular longevity 2018;2018:3987134
Oxidative medicine and cellular longevity 2018;2018:3987134
BMSCs pre-treatment ameliorates inflammation-related tissue destruction in LPS-induced rat DIC model.
Wang B, Wu S, Ma Z, Wang T, Yang C
Cell death & disease 2018 Oct 3;9(10):1024
Cell death & disease 2018 Oct 3;9(10):1024
Perturbing chondroitin sulfate proteoglycan signaling through LAR and PTPσ receptors promotes a beneficial inflammatory response following spinal cord injury.
Dyck S, Kataria H, Alizadeh A, Santhosh KT, Lang B, Silver J, Karimi-Abdolrezaee S
Journal of neuroinflammation 2018 Mar 20;15(1):90
Journal of neuroinflammation 2018 Mar 20;15(1):90
CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract.
Li LX, Labuda JC, Imai DM, Griffey SM, McSorley SJ
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 1;199(7):2547-2554
Journal of immunology (Baltimore, Md. : 1950) 2017 Oct 1;199(7):2547-2554
PI3Kγ Activates Integrin α4 and Promotes Immune Suppressive Myeloid Cell Polarization during Tumor Progression.
Foubert P, Kaneda MM, Varner JA
Cancer immunology research 2017 Nov;5(11):957-968
Cancer immunology research 2017 Nov;5(11):957-968
Therapeutic reversal of food allergen sensitivity by mature retinoic acid-differentiated dendritic cell induction of LAG3+CD49b-Foxp3- regulatory T cells.
Dawicki W, Li C, Town J, Zhang X, Gordon JR
The Journal of allergy and clinical immunology 2017 May;139(5):1608-1620.e3
The Journal of allergy and clinical immunology 2017 May;139(5):1608-1620.e3
A critical role for IRF5 in regulating allergic airway inflammation.
Byrne AJ, Weiss M, Mathie SA, Walker SA, Eames HL, Saliba D, Lloyd CM, Udalova IA
Mucosal immunology 2017 May;10(3):716-726
Mucosal immunology 2017 May;10(3):716-726
IL-33 contributes to sepsis-induced long-term immunosuppression by expanding the regulatory T cell population.
Nascimento DC, Melo PH, Piñeros AR, Ferreira RG, Colón DF, Donate PB, Castanheira FV, Gozzi A, Czaikoski PG, Niedbala W, Borges MC, Zamboni DS, Liew FY, Cunha FQ, Alves-Filho JC
Nature communications 2017 Apr 4;8:14919
Nature communications 2017 Apr 4;8:14919
Mesenchymal Stem Cell-Educated Macrophages Ameliorate LPS-Induced Systemic Response.
Hu Y, Qin C, Zheng G, Lai D, Tao H, Zhang Y, Qiu G, Ge M, Huang L, Chen L, Cheng B, Shu Q, Xu J
Mediators of inflammation 2016;2016:3735452
Mediators of inflammation 2016;2016:3735452
Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis.
Lim JY, Im KI, Lee ES, Kim N, Nam YS, Jeon YW, Cho SG
Scientific reports 2016 Jun 1;6:26851
Scientific reports 2016 Jun 1;6:26851
Neuropathic pain is constitutively suppressed in early life by anti-inflammatory neuroimmune regulation.
McKelvey R, Berta T, Old E, Ji RR, Fitzgerald M
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jan 14;35(2):457-66
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Jan 14;35(2):457-66
Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice.
Lalani AI, Moore CR, Luo C, Kreider BZ, Liu Y, Morse HC 3rd, Xie P
Journal of immunology (Baltimore, Md. : 1950) 2015 Jan 1;194(1):334-48
Journal of immunology (Baltimore, Md. : 1950) 2015 Jan 1;194(1):334-48
M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.
Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
The American journal of pathology 2015 Feb;185(2):420-31
The American journal of pathology 2015 Feb;185(2):420-31
IL-33-mediated protection against experimental cerebral malaria is linked to induction of type 2 innate lymphoid cells, M2 macrophages and regulatory T cells.
Besnard AG, Guabiraba R, Niedbala W, Palomo J, Reverchon F, Shaw TN, Couper KN, Ryffel B, Liew FY
PLoS pathogens 2015 Feb;11(2):e1004607
PLoS pathogens 2015 Feb;11(2):e1004607
TLR9 ligands induce S100A8 in macrophages via a STAT3-dependent pathway which requires IL-10 and PGE2.
Hsu K, Chung YM, Endoh Y, Geczy CL
PloS one 2014;9(8):e103629
PloS one 2014;9(8):e103629
Delayed wound healing due to increased interleukin-10 expression in mice with lymphatic dysfunction.
Kimura T, Sugaya M, Blauvelt A, Okochi H, Sato S
Journal of leukocyte biology 2013 Jul;94(1):137-45
Journal of leukocyte biology 2013 Jul;94(1):137-45
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine 2012 May;58(2):169-77
Cytokine 2012 May;58(2):169-77
Treatment failure of a TLR-7 agonist occurs due to self-regulation of acute inflammation and can be overcome by IL-10 blockade.
Lu H, Wagner WM, Gad E, Yang Y, Duan H, Amon LM, Van Denend N, Larson ER, Chang A, Tufvesson H, Disis ML
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5360-7
Journal of immunology (Baltimore, Md. : 1950) 2010 May 1;184(9):5360-7
Tr1 regulatory T cells induced by ConA pretreatment prevent mice from ConA-induced hepatitis.
Ye F, Yan S, Xu L, Jiang Z, Liu N, Xiong S, Wang Y, Chu Y
Immunology letters 2009 Feb 21;122(2):198-207
Immunology letters 2009 Feb 21;122(2):198-207
ST2 deficient mice display a normal host defense against pulmonary infection with Mycobacterium tuberculosis.
Wieland CW, van der Windt GJ, Florquin S, McKenzie AN, van der Poll T
Microbes and infection 2009 Apr;11(4):524-30
Microbes and infection 2009 Apr;11(4):524-30
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology 2008 Oct;125(2):272-9
Immunology 2008 Oct;125(2):272-9
Tumor-specific Th17-polarized cells eradicate large established melanoma.
Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP
Blood 2008 Jul 15;112(2):362-73
Blood 2008 Jul 15;112(2):362-73
Relative importance of T-cell subsets in monocytotropic ehrlichiosis: a novel effector mechanism involved in Ehrlichia-induced immunopathology in murine ehrlichiosis.
Ismail N, Crossley EC, Stevenson HL, Walker DH
Infection and immunity 2007 Sep;75(9):4608-20
Infection and immunity 2007 Sep;75(9):4608-20
Induction of antigen-specific regulatory T lymphocytes by human dendritic cells expressing the glucocorticoid-induced leucine zipper.
Hamdi H, Godot V, Maillot MC, Prejean MV, Cohen N, Krzysiek R, Lemoine FM, Zou W, Emilie D
Blood 2007 Jul 1;110(1):211-9
Blood 2007 Jul 1;110(1):211-9
Severe sepsis exacerbates cell-mediated immunity in the lung due to an altered dendritic cell cytokine profile.
Wen H, Hogaboam CM, Gauldie J, Kunkel SL
The American journal of pathology 2006 Jun;168(6):1940-50
The American journal of pathology 2006 Jun;168(6):1940-50
Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors.
Hajjar AM, Harvey MD, Shaffer SA, Goodlett DR, Sjöstedt A, Edebro H, Forsman M, Byström M, Pelletier M, Wilson CB, Miller SI, Skerrett SJ, Ernst RK
Infection and immunity 2006 Dec;74(12):6730-8
Infection and immunity 2006 Dec;74(12):6730-8
Sequential ELISA to profile multiple cytokines from small volumes.
Osuchowski MF, Siddiqui J, Copeland S, Remick DG
Journal of immunological methods 2005 Jul;302(1-2):172-81
Journal of immunological methods 2005 Jul;302(1-2):172-81
Nonlymphoid peritoneal cells suppress the T cell response to Mls.
Rosini L, Matlack R, Taylor J, Howell KF, Yeh K, Pennello A, Riggs JE
Immunobiology 2004;209(8):575-84
Immunobiology 2004;209(8):575-84
Selective macrophage suppression during sepsis.
Ellaban E, Bolgos G, Remick D
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Cellular immunology 2004 Sep-Oct;231(1-2):103-11
Attenuation of autoimmune disease in Fas-deficient mice by treatment with a cytotoxic benzodiazepine.
Bednarski JJ, Warner RE, Rao T, Leonetti F, Yung R, Richardson BC, Johnson KJ, Ellman JA, Opipari AW Jr, Glick GD
Arthritis and rheumatism 2003 Mar;48(3):757-66
Arthritis and rheumatism 2003 Mar;48(3):757-66
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Mouse IL-10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IL-10 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Mouse IL-10 Antibody. Recombinant Mouse IL-10 (Catalog # 417-ML) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-10 (2.5 ng/mL) is neutralized (green line) by increasing concen-trations of Rat Anti-Mouse IL-10 Monoclonal Antibody (Catalog # MAB417). The ND50 is typically 0.005-0.015 µg/mL.
