M011B

Bacchetta R, Gregori S, Serafini G, Sartirana C, Schulz U, Zino E, Tomiuk S, Jansen U, Ponzoni M, Paties CT, Fleischhauer K, Roncarolo MG
Haematologica 2010 Dec;95(12):2134-43

Type 1 regulatory T cells are associated with persistent split erythroid/lymphoid chimerism after allogeneic hematopoietic stem cell transplantation for thalassemia.

Serafini G, Andreani M, Testi M, Battarra M, Bontadini A, Biral E, Fleischhauer K, Marktel S, Lucarelli G, Roncarolo MG, Bacchetta R
Haematologica 2009 Oct;94(10):1415-26

Anti-ribosomal phosphoprotein autoantibody triggers interleukin-10 overproduction via phosphatidylinositol 3-kinase-dependent signalling pathways in lipopolysaccharide-activated macrophages.

Lee TP, Leu SJ, Huang JC, Song YC, Jhou RS, Tang SJ, Sun KH
Immunology 2009 May;127(1):91-102

Hepatitis C virus-specific reactivity of CD4+-lymphocytes in children born from HCV-infected women.

Della Bella S, Riva A, Tanzi E, Nicola S, Amendola A, Vecchi L, Nebbia G, Longhi R, Zanetti AR, Villa ML
Journal of hepatology 2005 Sep;43(3):394-402

Generation of three-dimensional pannus-like tissues in vitro from single cell suspensions of synovial fluid cells from arthritis patients.

Solomon S, Masilamani M, Mohanty S, Schwab JE, Boneberg EM, Illges H
Rheumatology international 2004 Mar;24(2):71-6

Long-term treatment with etanercept significantly reduces the number of proinflammatory cytokine-secreting peripheral blood mononuclear cells in patients with rheumatoid arthritis.

Schotte H, Schlüter B, Willeke P, Mickholz E, Schorat MA, Domschke W, Gaubitz M
Rheumatology (Oxford, England) 2004 Aug;43(8):960-4

Altered maturation of peripheral blood dendritic cells in patients with breast cancer.

Della Bella S, Gennaro M, Vaccari M, Ferraris C, Nicola S, Riva A, Clerici M, Greco M, Villa ML
British journal of cancer 2003 Oct 20;89(8):1463-72

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, Fietta A, Vitulo P, Pozzi E, Viganò M
Transplantation proceedings 2003 Jun;35(4):1523-6

Monocyte chemoattractant protein-1 levels in bronchoalveolar lavage fluid of lung-transplanted patients treated with tacrolimus as rescue treatment for refractory acute rejection.

Meloni F, Cascina A, Paschetto E, Marone Bianco A, Morosini M, Pellegrini C, Fietta A, Vitulo P, Pozzi E, Viganò M
Transplantation proceedings 2003 Jun;35(4):1523-6

Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.

Loffreda S, Muratori P, Muratori L, Mele L, Bianchi FB, Lenzi M
Journal of hepatology 2003 Feb;38(2):230-6

Enhanced monocyte Th1 cytokine production in HCV-infected cryoglobulinemic patients.

Loffreda S, Muratori P, Muratori L, Mele L, Bianchi FB, Lenzi M
Journal of hepatology 2003 Feb;38(2):230-6

The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.

Zarzaur BL, Wu Y, Fukatsu K, Johnson CD, Kudsk KA
Surgery 2002 Jan;131(1):59-65

The neuropeptide bombesin improves IgA-mediated mucosal immunity with preservation of gut interleukin-4 in total parenteral nutrition-fed mice.

Zarzaur BL, Wu Y, Fukatsu K, Johnson CD, Kudsk KA
Surgery 2002 Jan;131(1):59-65

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18

Ambient air particulates stimulate alveolar macrophages of smokers to promote differentiation of myeloid precursor cells.

Suwa T, Hogg JC, Vincent R, Mukae H, Fujii T, van Eeden SF
Experimental lung research 2002 Jan-Feb;28(1):1-18

Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.

McDowell MA, Marovich M, Lira R, Braun M, Sacks D
Infection and immunity 2002 Aug;70(8):3994-4001

Leishmania priming of human dendritic cells for CD40 ligand-induced interleukin-12p70 secretion is strain and species dependent.

McDowell MA, Marovich M, Lira R, Braun M, Sacks D
Infection and immunity 2002 Aug;70(8):3994-4001

Mistletoe lectin-1 increases tumor necrosis factor-alpha release in lipopolysaccharide-stimulated whole blood via inhibition of interleukin-10 production.

Boneberg EM, Hartung T
The Journal of pharmacology and experimental therapeutics 2001 Sep;298(3):996-1000

Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production.

Whittaker DS, Bahjat KS, Moldawer LL, Clare-Salzler MJ
Journal of immunology (Baltimore, Md. : 1950) 2000 Oct 15;165(8):4298-304

Autoregulation of human monocyte-derived dendritic cell maturation and IL-12 production by cyclooxygenase-2-mediated prostanoid production.

Whittaker DS, Bahjat KS, Moldawer LL, Clare-Salzler MJ
Journal of immunology (Baltimore, Md. : 1950) 2000 Oct 15;165(8):4298-304

Donor-specific stimulation of peripheral blood mononuclear cells from recipients of orthotopic liver transplants is associated, in the absence of rejection, with type-2 cytokine production.

Chung SW, Yoshida EM, Cattral MS, Hu Y, Gorczynski RM
Immunology letters 1998 Sep;63(2):91-6

Nitric oxide in Tanzanian children with malaria: inverse relationship between malaria severity and nitric oxide production/nitric oxide synthase type 2 expression.

Anstey NM, Weinberg JB, Hassanali MY, Mwaikambo ED, Manyenga D, Misukonis MA, Arnelle DR, Hollis D, McDonald MI, Granger DL
The Journal of experimental medicine 1996 Aug 1;184(2):557-67

Nitric oxide in Tanzanian children with malaria: inverse relationship between malaria severity and nitric oxide production/nitric oxide synthase type 2 expression.

Anstey NM, Weinberg JB, Hassanali MY, Mwaikambo ED, Manyenga D, Misukonis MA, Arnelle DR, Hollis D, McDonald MI, Granger DL
The Journal of experimental medicine 1996 Aug 1;184(2):557-67

Contribution of IL-1, CD14, and CD13 in the increased IL-6 production induced by in vitro monocyte-synoviocyte interactions.

Chomarat P, Rissoan MC, Pin JJ, Banchereau J, Miossec P
Journal of immunology (Baltimore, Md. : 1950) 1995 Oct 1;155(7):3645-52

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-IL-10 Monoclonal Antibody (12G8), Biotin (Product # M011B) and a 18 kDa band corresponding to Interleukin-10 was observed in Human rec. IL10 as compared to Human rec. IL6 which was used as a negative control. 250 ng of Human rec. IL10 (Lane 1), Human rec. IL6 (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Poly-HRP Streptavidin (Product # N200,1:20000 dilution) using the iBright FL 1500 (Product # A44115). Chemiluminescentdetection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Sandwich ELISA analysis of Rat anti-Human IL-10 biotinylated antibody (Product # M011B) was performed using the Thermo Scientific Human IL-10 Colorimetric ELISA kit (Product # EHIL10) by loading 50 µL per well of Human IL-10 recombinant protein (Product # SIL10) in triplicate at 600, 240, 96, 38.4, 15.36, and 0pg/mL across a 2 µg/mL Rat anti-Human IL-10 (Product # M010) pre-coated plate and incubated for 1.5 hours at room temperature. The plate was washed and incubated with 50 µL per well of Rat anti-Human IL-10 biotinylated antibody (Product # M011B) in triplicate at 0.3 µg/mL for 1.5 hours in conjunction with standards at room temperature. The plate was washed and incubated with 100 µL per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:18,000 for 15 minutes at room temperature. The plate was washed and incubated with 100 µL per well of TMB Substrate Solution (Product # N301) for 20 minutes at room temperature in the dark. The reaction was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Sandwich ELISA analysis of Rat anti-Human IL-10 biotinylated antibody (Product # M011B) was performed using the Thermo Scientific Human IL-10 Colorimetric ELISA kit (Product # EHIL10) by loading 50 µL per well of Human IL-10 recombinant protein (Product # SIL10) in triplicate at 600, 240, 96, 38.4, 15.36, and 0pg/mL across a 2 µg/mL Rat anti-Human IL-10 (Product # M010) pre-coated plate and incubated for 1.5 hours at room temperature. The plate was washed and incubated with 50 µL per well of Rat anti-Human IL-10 biotinylated antibody (Product # M011B) in triplicate at 0.3 µg/mL for 1.5 hours in conjunction with standards at room temperature. The plate was washed and incubated with 100 µL per well of Ultra Streptavidin-HRP (Product # N504) in all test wells at 1:18,000 for 15 minutes at room temperature. The plate was washed and incubated with 100 µL per well of TMB Substrate Solution (Product # N301) for 20 minutes at room temperature in the dark. The reaction was then stopped with 0.16M sulfuric acid. Absorbances were read on a spectrophotometer at 450-550 nm.
Conjugate: Biotin