RP1014

antibody from Boster Biological Technology

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

RP1014

Provider

Boster Biological Technology

Product name

Anti-Interleukin-10 IL10 Antibody

Antibody type

Polyclonal

Description

Polyclonal antibody for IL 10/IL10 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Human. IL 10/IL10 information: Molecular Weight: 20517 MW; Subcellular Localization: Secreted; Tissue Specificity: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.

Reactivity

Human

Host

Rabbit

Vial size

100μg/vial

Concentration

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

The role of glycogen synthase kinase 3 beta in brain injury induced by myocardial ischemia/reperfusion injury in a rat model of diabetes mellitus.

Zhao B, Gao WW, Liu YJ, Jiang M, Liu L, Yuan Q, Hou JB, Xia ZY
Neural regeneration research 2017 Oct;12(10):1632-1639

---

Women with Recurrent Miscarriage Have Decreased Expression of 25-Hydroxyvitamin D3-1α-Hydroxylase by the Fetal-Maternal Interface.

Wang LQ, Yan XT, Yan CF, Zhang XW, Hui LY, Xue M, Yu XW
PloS one 2016;11(12):e0165589

---

Foxp3 expression in CD4(+)CD25(+)Foxp3(+) regulatory T cells promotes development of colorectal cancer by inhibiting tumor immunity.

Zhu XW, Zhu HZ, Zhu YQ, Feng MH, Qi J, Chen ZF
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban 2016 Oct;36(5):677-682

---

T2 enhances in situ level of Foxp3+ regulatory cells and modulates inflammatory cytokines in Crohn's disease.

Li G, Ren J, Wang G, Gu G, Hu D, Ren H, Hong Z, Wu X, Liu S, Li J
International immunopharmacology 2014 Feb;18(2):244-8

---

Expression of interleukin-10 in patients with adenomyosis.

Wang F, Li H, Yang Z, Du X, Cui M, Wen Z
Fertility and sterility 2009 May;91(5):1681-5

Western blot

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Western blot analysis of IL-10 using anti- IL-10 antibody (RP1014). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane : Recombinant Human IL-10 Protein 0.5ng After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IL-10 antigen affinity purified polyclonal antibody (Catalog # RP1014) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-10 at approximately 19KD. The expected band size for IL-10 is at 19KD.

Additional image