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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [16]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF-217-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-10 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant Epstein-Barr virus IL-10 is observed and less than 20% cross-reactivity with recombinant mouse IL-10, recombinant feline IL-10, recombinant porcine IL-10, recombinant equine IL-10, and recombinant rat IL-10 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P22301- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Role of Interleukin-10 on Nasal Polypogenesis in Patients with Chronic Rhinosinusitis with Nasal Polyps.
Expression of Tim-1 in primary CNS lymphoma.
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Immune suppression and resistance mediated by constitutive activation of Wnt/β-catenin signaling in human melanoma cells.
IL-10 expression by primary tumor cells correlates with melanoma progression from radial to vertical growth phase and development of metastatic competence.
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
Leukocyte-derived IL-10 reduces subepithelial fibrosis associated with chronically inhaled endotoxin.
Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
Xu J, Han R, Kim DW, Mo JH, Jin Y, Rha KS, Kim YM
PloS one 2016;11(9):e0161013
PloS one 2016;11(9):e0161013
Expression of Tim-1 in primary CNS lymphoma.
Kishimoto W, Nishikori M, Arima H, Miyoshi H, Sasaki Y, Kitawaki T, Shirakawa K, Kato T, Imaizumi Y, Ishikawa T, Ohno H, Haga H, Ohshima K, Takaori-Kondo A
Cancer medicine 2016 Nov;5(11):3235-3245
Cancer medicine 2016 Nov;5(11):3235-3245
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Saulep-Easton D, Vincent FB, Quah PS, Wei A, Ting SB, Croce CM, Tam C, Mackay F
Leukemia 2016 Jan;30(1):163-72
Leukemia 2016 Jan;30(1):163-72
Immune suppression and resistance mediated by constitutive activation of Wnt/β-catenin signaling in human melanoma cells.
Yaguchi T, Goto Y, Kido K, Mochimaru H, Sakurai T, Tsukamoto N, Kudo-Saito C, Fujita T, Sumimoto H, Kawakami Y
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2110-7
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2110-7
IL-10 expression by primary tumor cells correlates with melanoma progression from radial to vertical growth phase and development of metastatic competence.
Itakura E, Huang RR, Wen DR, Paul E, Wünsch PH, Cochran AJ
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):801-9
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2011 Jun;24(6):801-9
Irritant-induced migration of Langerhans cells coincides with an IL-10-dependent switch to a macrophage-like phenotype.
Ouwehand K, Oosterhoff D, Breetveld M, Scheper RJ, de Gruijl TD, Gibbs S
The Journal of investigative dermatology 2011 Feb;131(2):418-25
The Journal of investigative dermatology 2011 Feb;131(2):418-25
Thymic stromal lymphopoietin from trophoblasts induces dendritic cell-mediated regulatory TH2 bias in the decidua during early gestation in humans.
Guo PF, Du MR, Wu HX, Lin Y, Jin LP, Li DJ
Blood 2010 Sep 23;116(12):2061-9
Blood 2010 Sep 23;116(12):2061-9
Evolving immunosuppressive microenvironment during human cervical carcinogenesis.
Kobayashi A, Weinberg V, Darragh T, Smith-McCune K
Mucosal immunology 2008 Sep;1(5):412-20
Mucosal immunology 2008 Sep;1(5):412-20
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
European journal of immunology 2008 Apr;38(4):1012-23
European journal of immunology 2008 Apr;38(4):1012-23
T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
Blood 2007 Jun 1;109(11):4856-64
Blood 2007 Jun 1;109(11):4856-64
Leukocyte-derived IL-10 reduces subepithelial fibrosis associated with chronically inhaled endotoxin.
Garantziotis S, Brass DM, Savov J, Hollingsworth JW, McElvania-TeKippe E, Berman K, Walker JK, Schwartz DA
American journal of respiratory cell and molecular biology 2006 Dec;35(6):662-7
American journal of respiratory cell and molecular biology 2006 Dec;35(6):662-7
Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de la Pintière CT, Genetet N, Patard JJ, Catros-Quemener V
Clinical and experimental immunology 2006 Dec;146(3):518-23
Clinical and experimental immunology 2006 Dec;146(3):518-23
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
Ogden CA, Pound JD, Batth BK, Owens S, Johannessen I, Wood K, Gregory CD
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23
Enhanced apoptotic cell clearance capacity and B cell survival factor production by IL-10-activated macrophages: implications for Burkitt's lymphoma.
Ogden CA, Pound JD, Batth BK, Owens S, Johannessen I, Wood K, Gregory CD
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23
Journal of immunology (Baltimore, Md. : 1950) 2005 Mar 1;174(5):3015-23
Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
Torrealba JR, Katayama M, Fechner JH Jr, Jankowska-Gan E, Kusaka S, Xu Q, Schultz JM, Oberley TD, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5753-64
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5753-64
Metastable tolerance to rhesus monkey renal transplants is correlated with allograft TGF-beta 1+CD4+ T regulatory cell infiltrates.
Torrealba JR, Katayama M, Fechner JH Jr, Jankowska-Gan E, Kusaka S, Xu Q, Schultz JM, Oberley TD, Hu H, Hamawy MM, Jonker M, Wubben J, Doxiadis G, Bontrop R, Burlingham WJ, Knechtle SJ
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5753-64
Journal of immunology (Baltimore, Md. : 1950) 2004 May 1;172(9):5753-64
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-10 by Simple WesternTM. Simple Western lane view shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10, loaded at 0.2 mg/mL. A specific band was detected for IL-10 at approximately 23 kDa (as indicated) using 10 µg/mL of Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-10 in Human Tonsil. IL-10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-10 in Human Tonsil. IL-10 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-217-NA). The ND50 is typically 0.2-1.0 µg/mL.
