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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4939 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4939, RRID:AB_1575291
- Product name
- GRB2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GRB2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references An analysis of the phosphoproteome of immune cell lines exposed to the immunomodulatory mycotoxin deoxynivalenol.
Coupling of Grb2 to Gab1 mediates hepatocyte growth factor-induced high intensity ERK signal required for inhibition of HepG2 hepatoma cell proliferation.
Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction.
Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed by using single-molecule analysis.
Nogueira da Costa A, Keen JN, Wild CP, Findlay JB
Biochimica et biophysica acta 2011 Jul;1814(7):850-7
Biochimica et biophysica acta 2011 Jul;1814(7):850-7
Coupling of Grb2 to Gab1 mediates hepatocyte growth factor-induced high intensity ERK signal required for inhibition of HepG2 hepatoma cell proliferation.
Kondo A, Hirayama N, Sugito Y, Shono M, Tanaka T, Kitamura N
The Journal of biological chemistry 2008 Jan 18;283(3):1428-36
The Journal of biological chemistry 2008 Jan 18;283(3):1428-36
Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction.
Martínez N, García-Domínguez CA, Domingo B, Oliva JL, Zarich N, Sánchez A, Gutiérrez-Eisman S, Llopis J, Rojas JM
Cellular signalling 2007 Nov;19(11):2277-85
Cellular signalling 2007 Nov;19(11):2277-85
Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed by using single-molecule analysis.
Morimatsu M, Takagi H, Ota KG, Iwamoto R, Yanagida T, Sako Y
Proceedings of the National Academy of Sciences of the United States of America 2007 Nov 13;104(46):18013-8
Proceedings of the National Academy of Sciences of the United States of America 2007 Nov 13;104(46):18013-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of GRB2 (arrow) using rabbit GRB2 polyclonal antibody (Cat # PAB4939). 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with GRB2 polyclonal antibody (Cat # PAB4939) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
