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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB6094 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6094, RRID:AB_1575290
- Product name
- GRB2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of GRB2.
- Antigen sequence
C-PRNYVTPVNRNV- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.
Lowenstein EJ, Daly RJ, Batzer AG, Li W, Margolis B, Lammers R, Ullrich A, Skolnik EY, Bar-Sagi D, Schlessinger J
Cell 1992 Aug 7;70(3):431-42
Cell 1992 Aug 7;70(3):431-42
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GRB2 polyclonal antibody (Cat # PAB6094) staining (2 ug/mL) of Jurkat lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- GRB2 polyclonal antibody (Cat # PAB6094) staining (0.3 ug/mL) of paraffin embedded human tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
