PAB28612

antibody from Abnova Corporation

Targeting: FLNA ABP-280, FLN, FLN1, OPD1, OPD2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28612

Provider

Abnova Corporation

Product name

FLNA polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant FLNA.

Antigen sequence

VICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRSQ
QLAPQYTYAQGGQQTWAPERPLVGVNGLDVTSLRP
FDLVIPFTIKKGEITGEVRMPSGKVAQPTITDNKD
GTVTVRYAPSEAGLHEMDIRYDNMHIPGSPLQFYV
DYVNCGHVT


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells), Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with FLNA polyclonal antibody (Cat # PAB28612) at 1:100-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with FLNA polyclonal antibody (Cat # PAB28612) at 1-4 ug/ml shows positivity in plasma membrane, cytoplasm & cytoskeleton (actin filaments).

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow with FLNA polyclonal antibody (Cat # PAB28612) shows strong cytoplasmic positivity in bone marrow poietic cells at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)