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LearnNB100-56723
antibody from Novus Biologicals
Targeting: TLR4
ARMD10, CD284, hToll, TLR-4
Western blot
Immunocytochemistry Immunoprecipitation Immunohistochemistry Flow cytometry Blocking/Neutralizing Functional assayAntibody data
- Antibody Data
- Antigen structure
- References [54]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [9]
- Blocking/Neutralizing [2]
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- Product number
- NB100-56723 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56723, RRID:AB_839180
- Product name
- Mouse Monoclonal TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Canine
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Nfa34810 Facilitates Nocardia farcinica Invasion of Host Cells and Stimulates Tumor Necrosis Factor Alpha Secretion through Activation of the NF-κB and Mitogen-Activated Protein Kinase Pathways via Toll-Like Receptor 4.
Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells.
Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF-α and IL-10 Production in Human Cutaneous Leishmaniasis.
Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation.
Toll-like receptors blocking restores in vitro microbicidal activity in latent tuberculosis-infected subjects.
The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation.
Substance P represents a novel first-line defense mechanism in the nose.
Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients.
ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells.
Platelets Toll-like receptor-4 in Crohns disease.
Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells.
Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection.
Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: Implications for a role of LBP in intracellular LPS-signaling.
Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine.
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
BMP-2 and TGF-β1 mediate biglycan-induced pro-osteogenic reprogramming in aortic valve interstitial cells.
Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
Repeated lipopolysaccharide stimulation promotes cellular senescence in human dental pulp stem cells (DPSCs).
Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4.
Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis.
Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production.
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules.
TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2.
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum.
Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation.
Human lymphatic endothelial cells express multiple functional TLRs.
Human lymphatic endothelial cells express multiple functional TLRs.
TLR-independent induction of human monocyte IL-1 by phosphoglycolipids from thermophilic bacteria.
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
The toll-like receptor pathway: a novel mechanism of infection-induced carcinogenesis of prostate epithelial cells.
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection.
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha.
In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes.
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.
Analysis of TLR4 polymorphic variants: new insights into TLR4/MD-2/CD14 stoichiometry, structure, and signaling.
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line.
Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses.
Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses.
NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages.
Evidence of Toll-like receptor molecules on human platelets.
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Ji X, Zhang X, Li H, Sun L, Hou X, Song H, Han L, Xu S, Qiu X, Wang X, Zheng N, Li Z
Infection and immunity 2020 Mar 23;88(4)
Infection and immunity 2020 Mar 23;88(4)
Mechanistic Roles of Matrilin-2 and Klotho in Modulating the Inflammatory Activity of Human Aortic Valve Cells.
The E, Yao Q, Zhang P, Zhai Y, Ao L, Fullerton DA, Meng X
Cells 2020 Feb 7;9(2)
Cells 2020 Feb 7;9(2)
Leishmania braziliensis Infection Enhances Toll-Like Receptors 2 and 4 Expression and Triggers TNF-α and IL-10 Production in Human Cutaneous Leishmaniasis.
Polari LP, Carneiro PP, Macedo M, Machado PRL, Scott P, Carvalho EM, Bacellar O
Frontiers in cellular and infection microbiology 2019;9:120
Frontiers in cellular and infection microbiology 2019;9:120
Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation.
Akhter N, Madhoun A, Arefanian H, Wilson A, Kochumon S, Thomas R, Shenouda S, Al-Mulla F, Ahmad R, Sindhu S
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;53(1):1-18
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2019;53(1):1-18
Toll-like receptors blocking restores in vitro microbicidal activity in latent tuberculosis-infected subjects.
Coelho da Silva FD, Covre LP, Stringari LL, Palaci M, Dietze R, Gomes DCO, Ribeiro-Rodrigues R
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 2019 Feb 1;23(2):212-218
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease 2019 Feb 1;23(2):212-218
The Synergy between Palmitate and TNF-α for CCL2 Production Is Dependent on the TRIF/IRF3 Pathway: Implications for Metabolic Inflammation.
Ahmad R, Al-Roub A, Kochumon S, Akther N, Thomas R, Kumari M, Koshy MS, Tiss A, Hannun YA, Tuomilehto J, Sindhu S, Rosen ED
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3599-3611
Journal of immunology (Baltimore, Md. : 1950) 2018 May 15;200(10):3599-3611
Substance P represents a novel first-line defense mechanism in the nose.
Larsson O, Tengroth L, Xu Y, Uddman R, Kumlien Georén S, Cardell LO
The Journal of allergy and clinical immunology 2018 Jan;141(1):128-136.e3
The Journal of allergy and clinical immunology 2018 Jan;141(1):128-136.e3
Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients.
Heftrig D, Sturm R, Oppermann E, Kontradowitz K, Jurida K, Schimunek L, Woschek M, Marzi I, Relja B
Mediators of inflammation 2017;2017:2608349
Mediators of inflammation 2017;2017:2608349
ADAMTS5 Deficiency in Calcified Aortic Valves Is Associated With Elevated Pro-Osteogenic Activity in Valvular Interstitial Cells.
Li F, Song R, Ao L, Reece TB, Cleveland JC Jr, Dong N, Fullerton DA, Meng X
Arteriosclerosis, thrombosis, and vascular biology 2017 Jul;37(7):1339-1351
Arteriosclerosis, thrombosis, and vascular biology 2017 Jul;37(7):1339-1351
Platelets Toll-like receptor-4 in Crohns disease.
Schmid W, Novacek G, Vogelsang H, Papay P, Primas C, Eser A, Panzer S
European journal of clinical investigation 2017 Feb;47(2):109-116
European journal of clinical investigation 2017 Feb;47(2):109-116
Mobilization of TLR4 Into Lipid Rafts by Aggregatibacter Actinomycetemcomitans in Gingival Epithelial Cells.
Imai H, Fujita T, Kajiya M, Ouhara K, Yoshimoto T, Matsuda S, Takeda K, Kurihara H
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;39(5):1777-1786
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2016;39(5):1777-1786
Activation of NLRP3 inflammasome in human neutrophils by Helicobacter pylori infection.
Pérez-Figueroa E, Torres J, Sánchez-Zauco N, Contreras-Ramos A, Alvarez-Arellano L, Maldonado-Bernal C
Innate immunity 2016 Feb;22(2):103-12
Innate immunity 2016 Feb;22(2):103-12
Lipopolysaccharide-binding protein is bound and internalized by host cells and colocalizes with LPS in the cytoplasm: Implications for a role of LBP in intracellular LPS-signaling.
Kopp F, Kupsch S, Schromm AB
Biochimica et biophysica acta 2016 Apr;1863(4):660-72
Biochimica et biophysica acta 2016 Apr;1863(4):660-72
Vaccination against canine leishmaniosis increases the phagocytic activity, nitric oxide production and expression of cell activation/migration molecules in neutrophils and monocytes.
Moreira ML, Costa-Pereira C, Alves ML, Marteleto BH, Ribeiro VM, Peruhype-Magalhães V, Giunchetti RC, Martins-Filho OA, Araújo MS
Veterinary parasitology 2016 Apr 15;220:33-45
Veterinary parasitology 2016 Apr 15;220:33-45
Porphyromonas gingivalis lipopolysaccharide inhibits trophoblast invasion in the presence of nicotine.
Komine-Aizawa S, Hirohata N, Aizawa S, Abiko Y, Hayakawa S
Placenta 2015 Jan;36(1):27-33
Placenta 2015 Jan;36(1):27-33
Toll-like receptor 3 expression and function in childhood idiopathic nephrotic syndrome.
Jamin A, Dehoux L, Dossier C, Fila M, Heming N, Monteiro RC, Deschênes G
Clinical and experimental immunology 2015 Dec;182(3):332-45
Clinical and experimental immunology 2015 Dec;182(3):332-45
BMP-2 and TGF-β1 mediate biglycan-induced pro-osteogenic reprogramming in aortic valve interstitial cells.
Song R, Fullerton DA, Ao L, Zheng D, Zhao KS, Meng X
Journal of molecular medicine (Berlin, Germany) 2015 Apr;93(4):403-12
Journal of molecular medicine (Berlin, Germany) 2015 Apr;93(4):403-12
Involvement of Toll-like receptors on Helicobacter pylori-induced immunity.
Käbisch R, Mejías-Luque R, Gerhard M, Prinz C
PloS one 2014;9(8):e104804
PloS one 2014;9(8):e104804
Repeated lipopolysaccharide stimulation promotes cellular senescence in human dental pulp stem cells (DPSCs).
Feng X, Feng G, Xing J, Shen B, Tan W, Huang D, Lu X, Tao T, Zhang J, Li L, Gu Z
Cell and tissue research 2014 May;356(2):369-80
Cell and tissue research 2014 May;356(2):369-80
Leishmania mexicana lipophosphoglycan activates ERK and p38 MAP kinase and induces production of proinflammatory cytokines in human macrophages through TLR2 and TLR4.
Rojas-Bernabé A, Garcia-Hernández O, Maldonado-Bernal C, Delegado-Dominguez J, Ortega E, Gutiérrez-Kobeh L, Becker I, Aguirre-Garcia M
Parasitology 2014 May;141(6):788-800
Parasitology 2014 May;141(6):788-800
Detailed pathologic evaluation on endomyocardial biopsy provides long-term prognostic information in patients with acute myocarditis.
Youn JC, Shim HS, Lee JS, Ji AY, Oh J, Hong N, Lee HS, Park S, Lee SH, Choi D, Chung N, Kang SM
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2014 May-Jun;23(3):139-44
Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology 2014 May-Jun;23(3):139-44
Activation of TLR4 signaling promotes gastric cancer progression by inducing mitochondrial ROS production.
Yuan X, Zhou Y, Wang W, Li J, Xie G, Zhao Y, Xu D, Shen L
Cell death & disease 2013 Sep 12;4:e794
Cell death & disease 2013 Sep 12;4:e794
Identification of lineage relationships and novel markers of blood and skin human dendritic cells.
Harman AN, Bye CR, Nasr N, Sandgren KJ, Kim M, Mercier SK, Botting RA, Lewin SR, Cunningham AL, Cameron PU
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
Journal of immunology (Baltimore, Md. : 1950) 2013 Jan 1;190(1):66-79
TAK-242 (resatorvid), a small-molecule inhibitor of Toll-like receptor (TLR) 4 signaling, binds selectively to TLR4 and interferes with interactions between TLR4 and its adaptor molecules.
Matsunaga N, Tsuchimori N, Matsumoto T, Ii M
Molecular pharmacology 2011 Jan;79(1):34-41
Molecular pharmacology 2011 Jan;79(1):34-41
TLR4-dependent induction of vascular adhesion molecule-1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A(2)alpha/cyclooxygenase-2.
Wu CY, Chi PL, Hsieh HL, Luo SF, Yang CM
Journal of cellular physiology 2010 May;223(2):480-91
Journal of cellular physiology 2010 May;223(2):480-91
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Pillay J, Ramakers BP, Kamp VM, Loi AL, Lam SW, Hietbrink F, Leenen LP, Tool AT, Pickkers P, Koenderman L
Journal of leukocyte biology 2010 Jul;88(1):211-20
Journal of leukocyte biology 2010 Jul;88(1):211-20
Toll-like receptor expression and function in subsets of human gammadelta T lymphocytes.
Pietschmann K, Beetz S, Welte S, Martens I, Gruen J, Oberg HH, Wesch D, Kabelitz D
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Scandinavian journal of immunology 2009 Sep;70(3):245-55
Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2.
Yang X, Fullerton DA, Su X, Ao L, Cleveland JC Jr, Meng X
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
Journal of the American College of Cardiology 2009 Feb 10;53(6):491-500
HIV-1 Tat protein suppresses cholangiocyte toll-like receptor 4 expression and defense against Cryptosporidium parvum.
O'Hara SP, Small AJ, Gajdos GB, Badley AD, Chen XM, Larusso NF
The Journal of infectious diseases 2009 Apr 15;199(8):1195-204
The Journal of infectious diseases 2009 Apr 15;199(8):1195-204
Stimulation of iNOS expression and apoptosis resistance in B-cell chronic lymphocytic leukemia (B-CLL) cells through engagement of Toll-like receptor 7 (TLR-7) and NF-kappaB activation.
Hammadi A, Billard C, Faussat AM, Kolb JP
Nitric oxide : biology and chemistry 2008 Sep;19(2):138-45
Nitric oxide : biology and chemistry 2008 Sep;19(2):138-45
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Human lymphatic endothelial cells express multiple functional TLRs.
Pegu A, Qin S, Fallert Junecko BA, Nisato RE, Pepper MS, Reinhart TA
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
Journal of immunology (Baltimore, Md. : 1950) 2008 Mar 1;180(5):3399-405
TLR-independent induction of human monocyte IL-1 by phosphoglycolipids from thermophilic bacteria.
Yang FL, Hua KF, Yang YL, Zou W, Chen YP, Liang SM, Hsu HY, Wu SH
Glycoconjugate journal 2008 Jul;25(5):427-39
Glycoconjugate journal 2008 Jul;25(5):427-39
Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry.
Burgener IA, Jungi TW
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
Veterinary immunology and immunopathology 2008 Jul 15;124(1-2):184-91
The toll-like receptor pathway: a novel mechanism of infection-induced carcinogenesis of prostate epithelial cells.
Kundu SD, Lee C, Billips BK, Habermacher GM, Zhang Q, Liu V, Wong LY, Klumpp DJ, Thumbikat P
The Prostate 2008 Feb 1;68(2):223-9
The Prostate 2008 Feb 1;68(2):223-9
TLR2 and TLR4 expression on the immune cells of tuberculous pleural fluid.
Prabha C, Rajashree P, Sulochana DD
Immunology letters 2008 Apr 15;117(1):26-34
Immunology letters 2008 Apr 15;117(1):26-34
A cellular micro-RNA, let-7i, regulates Toll-like receptor 4 expression and contributes to cholangiocyte immune responses against Cryptosporidium parvum infection.
Chen XM, Splinter PL, O'Hara SP, LaRusso NF
The Journal of biological chemistry 2007 Sep 28;282(39):28929-38
The Journal of biological chemistry 2007 Sep 28;282(39):28929-38
In vitro response to Candida albicans in cultures of whole human blood from young and aged donors.
Murciano C, Villamón E, Yáñez A, Murciano J, Mir A, O'Connor JE, Gozalbo D, Gil ML
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
FEMS immunology and medical microbiology 2007 Nov;51(2):327-35
Intracellular signaling mechanisms regulating toll-like receptor-mediated activation of eosinophils.
Wong CK, Cheung PF, Ip WK, Lam CW
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
American journal of respiratory cell and molecular biology 2007 Jul;37(1):85-96
Execution of macrophage apoptosis by PE_PGRS33 of Mycobacterium tuberculosis is mediated by Toll-like receptor 2-dependent release of tumor necrosis factor-alpha.
Basu S, Pathak SK, Banerjee A, Pathak S, Bhattacharyya A, Yang Z, Talarico S, Kundu M, Basu J
The Journal of biological chemistry 2007 Jan 12;282(2):1039-50
The Journal of biological chemistry 2007 Jan 12;282(2):1039-50
In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes.
Zanoni G, Navone R, Lunardi C, Tridente G, Bason C, Sivori S, Beri R, Dolcino M, Valletta E, Corrocher R, Puccetti A
PLoS medicine 2006 Sep;3(9):e358
PLoS medicine 2006 Sep;3(9):e358
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Shahrara S, Park CC, Temkin V, Jarvis JW, Volin MV, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
RANTES modulates TLR4-induced cytokine secretion in human peripheral blood monocytes.
Shahrara S, Park CC, Temkin V, Jarvis JW, Volin MV, Pope RM
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Journal of immunology (Baltimore, Md. : 1950) 2006 Oct 15;177(8):5077-87
Cell activation by ligands of the toll-like receptor and interleukin-1 receptor family depends on the function of the large-conductance potassium channel MaxiK in human macrophages.
Scheel O, Papavlassopoulos M, Blunck R, Gebert A, Hartung T, Zähringer U, Seydel U, Schromm AB
Infection and immunity 2006 Jul;74(7):4354-6
Infection and immunity 2006 Jul;74(7):4354-6
Analysis of TLR4 polymorphic variants: new insights into TLR4/MD-2/CD14 stoichiometry, structure, and signaling.
Rallabhandi P, Bell J, Boukhvalova MS, Medvedev A, Lorenz E, Arditi M, Hemming VG, Blanco JC, Segal DM, Vogel SN
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):322-32
Journal of immunology (Baltimore, Md. : 1950) 2006 Jul 1;177(1):322-32
A molecule that is associated with Toll-like receptor 4 and regulates its cell surface expression.
Konno K, Wakabayashi Y, Akashi-Takamura S, Ishii T, Kobayashi M, Takahashi K, Kusumoto Y, Saitoh S, Yoshizawa Y, Miyake K
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
Biochemical and biophysical research communications 2006 Jan 27;339(4):1076-82
High-avidity antitumor T-cell generation by toll receptor 8-primed, myeloid- derived dendritic cells is mediated by IL-12 production.
Xu S, Koldovsky U, Xu M, Wang D, Fitzpatrick E, Son G, Koski G, Czerniecki BJ
Surgery 2006 Aug;140(2):170-8
Surgery 2006 Aug;140(2):170-8
Expression of mRNA and proteins for toll-like receptors, associated molecules, defensins and LL-37 by SRIK-NKL, a CD8+ NK/T cell line.
Srivastava MD, Srivastava BI
Leukemia research 2005 Jul;29(7):813-20
Leukemia research 2005 Jul;29(7):813-20
Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses.
Schneeman TA, Bruno ME, Schjerven H, Johansen FE, Chady L, Kaetzel CS
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 1;175(1):376-84
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 1;175(1):376-84
Regulation of the polymeric Ig receptor by signaling through TLRs 3 and 4: linking innate and adaptive immune responses.
Schneeman TA, Bruno ME, Schjerven H, Johansen FE, Chady L, Kaetzel CS
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 1;175(1):376-84
Journal of immunology (Baltimore, Md. : 1950) 2005 Jul 1;175(1):376-84
NF-kappaB- and C/EBPbeta-driven interleukin-1beta gene expression and PAK1-mediated caspase-1 activation play essential roles in interleukin-1beta release from Helicobacter pylori lipopolysaccharide-stimulated macrophages.
Basak C, Pathak SK, Bhattacharyya A, Mandal D, Pathak S, Kundu M
The Journal of biological chemistry 2005 Feb 11;280(6):4279-88
The Journal of biological chemistry 2005 Feb 11;280(6):4279-88
Evidence of Toll-like receptor molecules on human platelets.
Cognasse F, Hamzeh H, Chavarin P, Acquart S, Genin C, Garraud O
Immunology and cell biology 2005 Apr;83(2):196-8
Immunology and cell biology 2005 Apr;83(2):196-8
Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2.
Lotz S, Aga E, Wilde I, van Zandbergen G, Hartung T, Solbach W, Laskay T
Journal of leukocyte biology 2004 Mar;75(3):467-77
Journal of leukocyte biology 2004 Mar;75(3):467-77
Toll-like receptor expression in human keratinocytes: nuclear factor kappaB controlled gene activation by Staphylococcus aureus is toll-like receptor 2 but not toll-like receptor 4 or platelet activating factor receptor dependent.
Mempel M, Voelcker V, Köllisch G, Plank C, Rad R, Gerhard M, Schnopp C, Fraunberger P, Walli AK, Ring J, Abeck D, Ollert M
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
The Journal of investigative dermatology 2003 Dec;121(6):1389-96
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR4 Antibody (HTA125) [NB100-56723] - Untransfected 293T cells probed with the monoclonal antibody against TLR4 (NB100-56723, Lane 1), TLR4 transfected 293T cells probed with the TLR4 monoclonal antibody (Lane 2), and with antibodies affinity-purified against the celiac peptide (Lane 3). TLR4 transfected 293T cells probed with TLR4 monoclonal (biotin) antibody (Lane 4) and probed first with affinity-purified anti-peptide antibodies, followed by an incubation with biotin-labelled anti-TLR4 monoclonal antibody (Lane 5). Human plasmocytoid dendritic cells probed with TLR4 monoclonal antibody (Lane 6), human monocytes probed with TLR4 monoclonal antibody (Lane 7) and with affinity-purified anti-peptide antibodies (Lane 8). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pmed.0030358) licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using this antibody. Secondary antibody goat anti-mouse IgG-PE at 0.25ug/10^6 cells. Both stable HEK293/hTLR4 and HEK293/Vector cell lines were equally stained.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using the PE conjugate of NB100-56727. Staining of TLR4 in human PBMCs using NB100-56062 at 0.5 ug/10^6 cells. The shaded histogram represents cells without anti-TLR4 antibody; green: isotype control; red: NB100-56062.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis of PE conjugate of NB100-56723. Cell surface TLR4 on ThP1 cells using TLR4 antibody at 2 ug/10^6 cells. The shaded histogram represents ThP1 cells only, green represents isotype control antibody, and red represents TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 in stable HEK293/hTLR4 cells using this antibody at 1ug/10^6 cells. Green: HEK293/Vector cells; red: HEK293/hTLR4 cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Cell surface analysis of TLR4 in stable HEK293/hTLR4 cells using NB100-56723 at 1 ug/10^6 cells. Secondary antibody Goat anti-Mouse IgG-PE at 0.25 ug/10^6 cells.
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Analysis of TLR4 using NB100-56723 on PBMCs at 2 ug/ 1x10^6 cells. M1 gate are unstained PBMC.
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using Azide/BSA FREE version of NB100-56727. Staining of human peripheral blood monocytes with Mouse anti Human CD284.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56727. Staining of TLR4 on PBMC using this antibody at 1 ug/10^6 cells. Green: isotype control; red: anti-TLR4 antibody.
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: TLR4 Antibody (HTA125) [NB100-56723] - Staining of U937 cells with mouse anti human CD284:RPE. Image using the PE form of this antibody.
Supportive validation
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- Novus Biologicals (provider)
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- Block/Neutralize: TLR4 Antibody (HTA125) [NB100-56723] - Validation of the neutralizing effect of this antibody on TLR4 activity. The TLR4/MD-2/CD14/IL-8 Prom/LUCPorter HeLa cells were plated in 96-well white plates at 1 x 10^5 cells/well. After 16 h, cells were preincubated with different amounts of this antibody for 1 h and then stimulated with 10 ng/ml LPS for 6 h. Luciferase activity was then directly analyzed using the one-step luciferase reporter assay reagent. Data Summary: It inhibited LPS-mediated TLR4 activity in the TLR4/MD-2/CD14/IL-8 Prom/LUCPorter cell line. Image using the Azide Free format of this antibody.
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- Novus Biologicals (provider)
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- Experimental details
- Block/Neutralize: TLR4 Antibody (HTA125) [NB100-56723] - Human monocyte-derived DCs were pre-incubated wit TLR-neutralizing antibodies including TLR4 antibody (NB100-56723) for 1 h. Afterwards, DCs were infected with H. pylori G27 (MOI 5) and the levels of the costimulatory molecule CD86 and the maturation marker CD83 were analyzed by FACS on CD11c+ cells 24 h post-infection. Data are presented as mean +/- SD of five independent experiments. *p
