312802

antibody from BioLegend

Targeting: TLR4 ARMD10, CD284, hToll, TLR-4

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

312802

Provider

BioLegend

Proper citation

BioLegend Cat#312802, RRID:AB_314951

Product name

Purified anti-human CD284 (TLR4)

Antibody type

Monoclonal

Antigen

Ba/F3 cell line expressing human TLR4

Description

Toll-like receptors are type I transmembrane signaling receptors. They are primordial pathogen-recognition proteins that function as sentinels for the innate immune system. TLR4, also known as CD284, is a 110 kD protein which is expressed on monocytes/macrophages, endothelial cells, and at low levels on B cells and granulocytes. In association with a secretory molecule, MD2, TLR4 has been recognized as critical for host recognition of bacterial LPS. HTA125 antibody is useful for flow cytometric analysis and is able to block LPS-induced cytokine production.

Reactivity

Human, Guinea Pig, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

The antibody solution should be stored undiluted at 4°C.

References

Submitted references

Toll-like receptor 4 mediates induction of the Bcl10-NFkappaB-interleukin-8 inflammatory pathway by carrageenan in human intestinal epithelial cells.

Bhattacharyya S, Gill R, Chen ML, Zhang F, Linhardt RJ, Dudeja PK, Tobacman JK
The Journal of biological chemistry 2008 Apr 18;283(16):10550-8

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High-mobility group box 1 activates integrin-dependent homing of endothelial progenitor cells.

Chavakis E, Hain A, Vinci M, Carmona G, Bianchi ME, Vajkoczy P, Zeiher AM, Chavakis T, Dimmeler S
Circulation research 2007 Feb 2;100(2):204-12

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood monocytes stained with HTA125 PE

Conjugate

Unconjugated