ALX-804-430-C100

antibody from Enzo Life Sciences

Targeting: TLR4 ARMD10, CD284, hToll, TLR-4

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

ALX-804-430-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-804-430-C100, RRID:AB_2052692

Product name

Toll-like receptor 4/MD-2 (mouse) monoclonal antibody (MTS510)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Mouse

Host

Rat

Isotype

IgG

Antibody clone number

MTS510

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

The role of TLR2 in the inflammatory activation of mouse fibroblasts by human antiphospholipid antibodies.

Satta N, Dunoyer-Geindre S, Reber G, Fish RJ, Boehlen F, Kruithof EK, de Moerloose P
Blood 2007 Feb 15;109(4):1507-14

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Synergy and cross-tolerance between toll-like receptor (TLR) 2- and TLR4-mediated signaling pathways.

Sato S, Nomura F, Kawai T, Takeuchi O, Mühlradt PF, Takeda K, Akira S
Journal of immunology (Baltimore, Md. : 1950) 2000 Dec 15;165(12):7096-101

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Cutting edge: endotoxin tolerance in mouse peritoneal macrophages correlates with down-regulation of surface toll-like receptor 4 expression.

Nomura F, Akashi S, Sakao Y, Sato S, Kawai T, Matsumoto M, Nakanishi K, Kimoto M, Miyake K, Takeda K, Akira S
Journal of immunology (Baltimore, Md. : 1950) 2000 Apr 1;164(7):3476-9

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Cutting edge: cell surface expression and lipopolysaccharide signaling via the toll-like receptor 4-MD-2 complex on mouse peritoneal macrophages.

Akashi S, Shimazu R, Ogata H, Nagai Y, Takeda K, Kimoto M, Miyake K
Journal of immunology (Baltimore, Md. : 1950) 2000 Apr 1;164(7):3471-5

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

FACS analysis using MAb to to Toll-like Receptor 4/MD-2 (mouse) (MTS510) (Prod. No. ALX-804-430). Method: Mouse BaF3 Cells (5 x 10E5) expressing surface mTLR4 alone or together with the accessory protein MD-2 or BaF3 parental cells, were incubated on ice for 30min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 1µg/ml of MAb to Toll-like Receptor 4/MD-2 (mouse) (MTS510). After washing in FACS buffer, FITC-conjugated antibody to rat IgG (Prod. No. ALX-211-051) was added. Cells were incubated on ice for 30 min., washed and then analyzed by flow cytometry.