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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-55666 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S100A14 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: GQCRSANAED AQEFSDVERA IETLIKNFHQ YSVEGGKETL TPSELRDLVT QQLPHLMPSN CGLEEKIANL GSCNDSKLEF RSFWELIGEA Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 37%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.10 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A14 in control (vector only transfected HEK293T lysate) and S100A14 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a S100A14 Polyclonal Antibody (Product # PA5-55666).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of S100A14 in human cell line MCF7 shows positivity in plasma membrane, cell junctions & nucleus but excluded from the nucleoli. Samples were probed using a S100A14 Polyclonal Antibody (Product # PA5-55666).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of S100A14 in human cell line MCF7 using a S100A14 Polyclonal Antibody (Product # PA5-55666) shows localization to nuclear bodies, cytosol and cell junctions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of S100A14 in human esophagus and kidney tissues using S100A14 Polyclonal Antibody (Product # PA5-55666). Corresponding S100A14 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of S100A14 in human kidney using S100A14 Polyclonal Antibody (Product # PA5-55666) shows low expression as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of S100A14 in human esophagus using S100A14 Polyclonal Antibody (Product # PA5-55666) shows high expression.
