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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00004999-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004999-M01, RRID:AB_425574
- Product name
- ORC2L monoclonal antibody (M01), clone 3E11-1G5
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ORC2L.
- Antigen sequence
MSKPELKEDKMLEVHFVGDDDVLNHILDREGGAKL
KKERAQLLVNPKKIIKKPEYDLEEDDQEVLKDQNY
VEIMGRDVQESLKNGSATGGGNKVYSFQNRKHSEK
MAKLASELAKTPQKSVSFSLKNDPEITINVPQSSK
GHSASDKVQPKNNDKSEFLSTAPRSLRKRLIVPRS
HSDSESEYSASNSEDDEGVAQEHEEDTNAVIFSQK
IQAQNRVVSAPVGKETPSKRMKRDKTSDLVEEYFE
AHSSSKVLTSDRTLQKLKRAKLDQQTLRNLLSKVS
PSFSAELKQLNQQYEKLFHKWMLQLHLGFNIVLYG
LGSKRDLLERFRTTMLQDSIHVVINGFFPGISVKS
VLNSITEEVLDHMGTFRSILDQLDWIVNKFKEDSS
LELFLLIHNLDSQMLRGEKSQQIIGQLSSLHNIYL
IASIDHLNAPLMWDHAKQSLFNWLWYETTTYSPYT
EETSYENSLLVKQSGSLPLSSLTHVLRSLTPNARG
IFRLLIKYQLDNQDNPSYIGLSFQDFYQQCREAFL
VNSDLTLRAQLTEFRDHKLIRTKKGTDGVEYLLIP
VDNGTLTDFLEKEEEEA- Isotype
- IgG
- Antibody clone number
- 3E11-1G5
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references RBM3-regulated genes promote DNA integrity and affect clinical outcome in epithelial ovarian cancer.
Ehlén Å, Nodin B, Rexhepaj E, Brändstedt J, Uhlén M, Alvarado-Kristensson M, Pontén F, Brennan DJ, Jirström K
Translational oncology 2011 Aug;4(4):212-21
Translational oncology 2011 Aug;4(4):212-21
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ORC2L monoclonal antibody (M01), clone 3E11-1G5 Western Blot analysis of ORC2L expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ORC2L monoclonal antibody (M01), clone 3E11-1G5. Western Blot analysis of ORC2L expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ORC2L is approximately 0.3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to ORC2L on formalin-fixed paraffin-embedded human spleen tissue.[antibody concentration 5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
