16620-1-AP

antibody from Invitrogen Antibodies

Targeting: INTS3 C1orf60, FLJ21919, INT3, SOSS-A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

16620-1-AP

Provider

Invitrogen Antibodies

Product name

INTS3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: YHLLYYLRA SKAAAGKMNL YESFAQATQL GDLHTCLMMD MKACQEDDVR LLCHLTPSIY TEFPDETLRS GELLNMIVAV IDSAQLQELV CHVMMGNLVM FRKDSVLNIL IQSLDWETFE QYCAWQLFLA HNIPLETIIP ILQHLKYKEH PEALSCLLLQ LRREKPSEEM VKMVLSRPCH PDDQFTTSIL RHWCMKHDEL LAEHIKSLLI KNNSLPRKRQ SLRSSSSKLA QLTLEQILEH LDNLRLNLTN TKQNFFSQTP ILQALQHVQA SCDEAHKMKF SDLFSLAEEY EDSSTKPPKS RRKAALSSPR SRKNATQPPN AEEESGSSSA SEEEDTKPKP TKRKRKGSSA VGSDSD (91-445 aa encoded by BC025254)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16620-1-AP (INTS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16620-1-AP (INTS3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 16620-1-AP (INTS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 16620-1-AP (INTS3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse testis tissue were subjected to SDS PAGE followed by western blot with 16620-1-AP (INTS3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 16620-1-AP) (INTS3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HeLa cells using 16620-1-AP (INTS3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-INTS3 (IP:16620-1-AP, 4ug; Detection:16620-1-AP 1:500) with mouse testis tissue lysate 4000ug.