Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- A300-533A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBAP2L/NICE4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human UBAP2L/NICE-4 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Jurkat, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBAP2L/NICE-4 antibody (Product # A300-533A; lot A300-533A-3) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human UBAP2L/NICE4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE4 antibody A300-533A (lot A300-533A-2) used for IP at 6 µg per reaction. UBAP2L/NICE4 was also immunoprecipitated by rabbit anti-UBAP2L/NICE4 antibody A300-534A. For blotting immunoprecipitated UBAP2L/NICE4, A300-533A was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human UBAP2L/NICE4 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE4 antibody A300-533A (lot A300-533A-2) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human UBAP2L/NICE-4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBAP2L/NICE-4 antibody (Product # A300-533A; lot A300-533A-3) used for IP at 6 µg per reaction. UBAP2L/NICE-4 was also immunoprecipitated by a previous lot of this antibody (lot A300-533A-2). For blotting immunoprecipitated UBAP2L/NICE-4, Product # A300-533A was used at 0.04 µg/mL. Detection: Chemiluminescence with an exposure time of 1 second.