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LearnH00004790-M02
antibody from Abnova Corporation
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Proximity ligation assay [1]
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Validation data
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- Product number
- H00004790-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004790-M02, RRID:AB_530143
- Product name
- NFKB1 monoclonal antibody (M02), clone 4A11
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant NFKB1.
- Antigen sequence
MDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASS
PVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSG
VETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGP
LEGKI- Isotype
- IgG
- Antibody clone number
- 4A11
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate(105.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1. HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
