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Learn100-4164
antibody from Rockland Immunochemicals, Inc.
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 100-4164 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-4164, RRID:AB_2153394
- Product name
- Anti-NFKB p50 (NFKB1) (RABBIT) Antibody - 100-4164
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references The eukaryotic translation initiation factor eIF4E is a direct transcriptional target of NF-κB and is aberrantly regulated in acute myeloid leukemia.
Development of a sensitive multi-well colorimetric assay for active NFkappaB.
Hariri F, Arguello M, Volpon L, Culjkovic-Kraljacic B, Nielsen TH, Hiscott J, Mann KK, Borden KL
Leukemia 2013 Oct;27(10):2047-55
Leukemia 2013 Oct;27(10):2047-55
Development of a sensitive multi-well colorimetric assay for active NFkappaB.
Renard P, Ernest I, Houbion A, Art M, Le Calvez H, Raes M, Remacle J
Nucleic acids research 2001 Feb 15;29(4):E21
Nucleic acids research 2001 Feb 15;29(4):E21
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h. Washes with TBS preceded color development.
- Validation comment
- Western Blot
