616704
antibody from BioLegend
Targeting: NFKB1
KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50
Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 616704 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#616704, RRID:AB_493279
- Product name
- Alexa Fluor® 488 anti-NF-êB p50 [4D1]
- Antibody type
- Monoclonal
- Antigen
- recombinant full-length human NF-κB p50
- Description
- NF-κB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p50 is bound (with p65) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF-κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-κB p50 and has been shown to be useful for Western blotting.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Submitted references STAT-3 activates NF-kappaB in chronic lymphocytic leukemia cells.
Requirements for two proximal NF-kappaB binding sites and IkappaB-zeta in IL-17A-induced human beta-defensin 2 expression by conducting airway epithelium.
Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells.
Liu Z, Hazan-Halevy I, Harris DM, Li P, Ferrajoli A, Faderl S, Keating MJ, Estrov Z
Molecular cancer research : MCR 2011 Apr;9(4):507-15
Molecular cancer research : MCR 2011 Apr;9(4):507-15
Requirements for two proximal NF-kappaB binding sites and IkappaB-zeta in IL-17A-induced human beta-defensin 2 expression by conducting airway epithelium.
Kao CY, Kim C, Huang F, Wu R
The Journal of biological chemistry 2008 May 30;283(22):15309-18
The Journal of biological chemistry 2008 May 30;283(22):15309-18
Requirement for both JAK-mediated PI3K signaling and ACT1/TRAF6/TAK1-dependent NF-kappaB activation by IL-17A in enhancing cytokine expression in human airway epithelial cells.
Huang F, Kao CY, Wachi S, Thai P, Ryu J, Wu R
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6504-13
Journal of immunology (Baltimore, Md. : 1950) 2007 Nov 15;179(10):6504-13
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Hela cells were stained with Alexa Fluor® 488 anti-NF-kB p50 (4D1) mouse mAb and examined on a fluorescent scope.
- Conjugate
- Green dye