616706

antibody from BioLegend

Targeting: NFKB1 KBF1, NF-kappaB, NF-kB1, NFkappaB, NFKB-p50, p105, p50

Immunocytochemistry

Antibody data

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Validation data

Product number: 616706 - Provider product page
Provider: BioLegend
Proper citation: BioLegend Cat#616706, RRID:AB_2130662
Product name: FITC anti-NF-B p50
Antibody type: Monoclonal
Antigen: recombinant full-length human NF-κB p50
Description: NF-κB/p50 (nuclear factor kappa light chain enhancer in B cells p50, NF-κB1) is a member of the Rel/dorsal family. This ubiquitously expressed nuclear protein is one subunit of the NF-kappa B complex consisting of a 65 kD transactivating subunit and a 50 kD DNA binding subunit. Both subunits are derived from larger precursor proteins. NF-κB acts as a transcriptional activator and was originally identified as an activator of kappa light chain in B cells. NF-κB/p50 is bound (with p65) to IκB inhibitor in cytoplasm in inactive form. Phosphorylation of IκB releases and NF-κB which goes to the nucleus to activate gene expression. NF-κB can be activated with LPS, TNF-α, phorbol ester, or IL-1. The 4D1 monoclonal antibody reacts with human NF-κB p50 and has been shown to be useful for Western blotting.
Reactivity: Human
Host: Mouse
Conjugate: Green dye
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/ml
Storage: The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

NFKB1 protein structure - 616706 shown in red.