Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5458 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal HAX-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human HAX-1 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references PELO negatively regulates HER receptor signalling and metastasis.
Pedersen K, Canals F, Prat A, Tabernero J, Arribas J
Oncogene 2014 Feb 27;33(9):1190-7
Oncogene 2014 Feb 27;33(9):1190-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Detection of Human HAX-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAX-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5458) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HAX-1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.