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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00079800-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00079800-M01, RRID:AB_2305450
- Product name
- Mouse Monoclonal ALS2CR8 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ALS2CR8 - amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 8
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of ALS2CR8 over-expressed 293 cell line, cotransfected with ALS2CR8 Validated Chimera RNAi ( Cat # H00079800-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALS2CR8 monoclonal antibody (M01), clone 2A3 (Cat # H00079800-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of ALS2CR8 expression in transfected 293T cell line by ALS2CR8 monoclonal antibody (M01), clone 2A3.Lane 1: ALS2CR8 transfected lysate(79.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ALS2CR8 Antibody (2A3) [H00079800-M01] - ALS2CR8 monoclonal antibody (M01), clone 2A3 Analysis of ALS2CR8 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: ALS2CR8 Antibody (2A3) [H00079800-M01] - Detection limit for recombinant GST tagged ALS2CR8 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of monoclonal antibody to ALS2CR8 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ALS2CR8 Antibody (2A3) [H00079800-M01] - Analysis of monoclonal antibody to ALS2CR8 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ug/ml.
