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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 16702-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP20A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: SGGGSVSEN HMREKLYENG VTDSLKSNFA LLLKLSEELL DKWLSYPETQ HVPLSQHMLG FAMKSVTQMV MGSTFEDDQE VIRFQKNHGT VWSEIGKGFL DGSLDKNMTR KKQYEDALMQ LESVLRNIIK ERKGRNFSQH IFIDSLVQGN LNDQQILEDS MIFSLASCII TAKLCTWAIC FLTTSEEVQK KLYEEINQVF GNGPVTPEKI EQLRYCQHVL CETVRTAKLT PVSAQFQDIE GKIDRFIIPR ETLVLYALGV VLQDPNTWPS PHKFDPDRFD DELVMKTFSS LGFSGTQECP ELRFAYMVTT VLLSVLVKRL HLLSVEGQVI ETKYELVTSS REEAWITVSK RY (112-462 aa encoded by BC020616)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 16702-1-AP (CYP20A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16702-1-AP (CYP20A1 antibody) at dilution of 1:200 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CYP20A1 (IP:16702-1-AP, 4ug; Detection:16702-1-AP 1:300) with MCF-7 cells lysate 2400 ug.
