NBP1-86863

antibody from Novus Biologicals

Targeting: EZR VIL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-86863

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-86863, RRID:AB_11010029

Product name

Rabbit Polyclonal Ezrin Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Specificity of human, mouse, rat Ezrin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Incident urothelial cancer in the Malmö Diet and Cancer Study: cohort characteristics and further validation of ezrin as a prognostic biomarker.

Wennersten C, Andersson G, Boman K, Nodin B, Gaber A, Jirström K
Diagnostic pathology 2014 Oct 3;9:189

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Reduced expression of ezrin in urothelial bladder cancer signifies more advanced tumours and an impaired survival: validatory study of two independent patient cohorts.

Andersson G, Wennersten C, Gaber A, Boman K, Nodin B, Uhlén M, Segersten U, Malmström PU, Jirström K
BMC urology 2014 May 12;14:36

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in human cell line A-431.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ezrin Antibody [NBP1-86863] - Analysis in human placenta tissue.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human stomach (upper) shows strong cytoplasmic positivity in parietal cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human placenta shows high expression.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human skeletal muscle shows low expression as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Analysis in human placenta and skeletal muscle tissues. Corresponding Ezrin RNA-seq data are presented for the same tissues.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human lymph node shows strong membranous positivity in germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human placenta shows strong membranous positivity in trophoblastic cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody [NBP1-86863] - Staining of human small intestine shows strong positivity in apical membrane in glandular cells.