Antibody data
- Antibody Data
- Antigen structure
- References [42]
- Comments [0]
- Validations
- Immunocytochemistry [5]
- Immunohistochemistry [3]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-13859 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Ezrin Monoclonal Antibody (3C12), Biotin
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Description
- MA5-13862 targets Ezrin in IHC (P), ICC, IP, IF, and WB applications and shows reactivity with Bovine, Hamster, Human, Marsupial, mouse, Non-human primate, and Rat samples. The MA5-13862 immunogen is a recombinant fragment of human ezrin corresponding to aa 362-585.
- Reactivity
- Human, Mouse, Rat, Bovine, Hamster
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- 3C12
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references Fascin-1, ezrin and paxillin contribute to the malignant progression and are predictors of clinical prognosis in laryngeal squamous cell carcinoma.
A-kinase anchoring proteins regulate compartmentalized cAMP signaling in airway smooth muscle.
Molecular basis of early epithelial response to streptococcal exotoxin: role of STIM1 and Orai1 proteins.
Phosphorylation of EBP50 negatively regulates β-PIX-dependent Rac1 activity in anoikis.
Role of CD44 in the organization of keratinocyte pericellular hyaluronan.
The effect of radixin knockdown on the expression and efflux function of MRP2 in SGC-7901 cells.
Activation of Ras requires the ERM-dependent link of actin to the plasma membrane.
Expression of ezrin in human embryonic, fetal, and normal adult tissues.
ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells.
Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma.
The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug transporters in mouse brain.
p35 is required for CDK5 activation in cellular senescence.
Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma.
Ezrin mediates c-Myc actions in prostate cancer cell invasion.
Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in mice.
A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays.
Establishment and characterization of 4 new human pancreatic cancer cell lines: evidences of different tumor phenotypes.
Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
Involvement of CD44v6 in InlB-dependent Listeria invasion.
Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma.
Slit diaphragms contain tight junction proteins.
Expression of ezrin is associated with invasion and dedifferentiation of hepatitis B related hepatocellular carcinoma.
Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells.
Nestin expression in repopulating mesangial cells promotes their proliferation.
Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin.
Adhesion molecules as prognostic markers in pancreatic adenocarcinoma.
Ezrin and radixin both regulate the apical membrane localization of ABCC2 (MRP2) in human intestinal epithelial Caco-2 cells.
A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135.
Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type.
Ezrin in osteosarcoma: comparison between conventional high-grade and central low-grade osteosarcoma.
Androgen induction of prostate cancer cell invasion is mediated by ezrin.
Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma.
Prognostic impact of immunohistochemical expression of ezrin in highly malignant soft tissue sarcomas.
Ezrin expression in prostate cancer and benign prostatic tissue.
Stromal cells in hemangioblastoma: neuroectodermal differentiation and morphological similarities to ependymoma.
Myocilin is expressed in the glomerulus of the kidney and induced in mesangioproliferative glomerulonephritis.
Expression of ezrin in prostatic intraepithelial neoplasia.
Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine.
Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients.
Gene expression profiling of androgen deficiency predicts a pathway of prostate apoptosis that involves genes related to oxidative stress.
Thyroid hormone-deficient period prior to the onset of hearing is associated with reduced levels of beta-tectorin protein in the tectorial membrane: implication for hearing loss.
ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse.
Gao W, Zhang C, Feng Y, Chen G, Wen S, Huangfu H, Wang B
PloS one 2012;7(11):e50710
PloS one 2012;7(11):e50710
A-kinase anchoring proteins regulate compartmentalized cAMP signaling in airway smooth muscle.
Horvat SJ, Deshpande DA, Yan H, Panettieri RA, Codina J, DuBose TD Jr, Xin W, Rich TC, Penn RB
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Sep;26(9):3670-9
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2012 Sep;26(9):3670-9
Molecular basis of early epithelial response to streptococcal exotoxin: role of STIM1 and Orai1 proteins.
Usmani SM, von Einem J, Frick M, Miklavc P, Mayenburg M, Husmann M, Dietl P, Wittekindt OH
Cellular microbiology 2012 Mar;14(3):299-315
Cellular microbiology 2012 Mar;14(3):299-315
Phosphorylation of EBP50 negatively regulates β-PIX-dependent Rac1 activity in anoikis.
Chen JY, Lin YY, Jou TS
Cell death and differentiation 2012 Jun;19(6):1027-37
Cell death and differentiation 2012 Jun;19(6):1027-37
Role of CD44 in the organization of keratinocyte pericellular hyaluronan.
Pasonen-Seppänen S, Hyttinen JM, Rilla K, Jokela T, Noble PW, Tammi M, Tammi R
Histochemistry and cell biology 2012 Jan;137(1):107-20
Histochemistry and cell biology 2012 Jan;137(1):107-20
The effect of radixin knockdown on the expression and efflux function of MRP2 in SGC-7901 cells.
He XJ, Wang WR, Zhang Y, Yang Q
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2012 Aug 15;46(5):426-34
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences 2012 Aug 15;46(5):426-34
Activation of Ras requires the ERM-dependent link of actin to the plasma membrane.
Sperka T, Geissler KJ, Merkel U, Scholl I, Rubio I, Herrlich P, Morrison HL
PloS one 2011;6(11):e27511
PloS one 2011;6(11):e27511
Expression of ezrin in human embryonic, fetal, and normal adult tissues.
Xie JJ, Zhang FR, Tao LH, Lü Z, Xu XE, Jian-Shen, Xu LY, Li EM
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 Nov;59(11):1001-8
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2011 Nov;59(11):1001-8
ERM stable knockdown by siRNA reduced in vitro migration and invasion of human SGC-7901 cells.
Ou-Yang M, Liu HR, Zhang Y, Zhu X, Yang Q
Biochimie 2011 May;93(5):954-61
Biochimie 2011 May;93(5):954-61
Expression of the membrane-cytoskeletal linker Ezrin in salivary gland adenoid cystic carcinoma.
Wang YY, Chen WL, Huang ZQ, Yang ZH, Zhang B, Wang JG, Li HG, Li JS
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 2011 Jul;112(1):96-104
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 2011 Jul;112(1):96-104
The effect of sphingomyelin synthase 2 (SMS2) deficiency on the expression of drug transporters in mouse brain.
Zhang Y, Dong J, Zhu X, Wang W, Yang Q
Biochemical pharmacology 2011 Aug 1;82(3):287-94
Biochemical pharmacology 2011 Aug 1;82(3):287-94
p35 is required for CDK5 activation in cellular senescence.
Mao D, Hinds PW
The Journal of biological chemistry 2010 May 7;285(19):14671-80
The Journal of biological chemistry 2010 May 7;285(19):14671-80
Expression and clinical significance of Ezrin and E-cadherin in esophageal squamous cell carcinoma.
Zhai JW, Yang XG, Yang FS, Hu JG, Hua WX
Chinese journal of cancer 2010 Mar;29(3):317-20
Chinese journal of cancer 2010 Mar;29(3):317-20
Ezrin mediates c-Myc actions in prostate cancer cell invasion.
Chuan YC, Iglesias-Gato D, Fernandez-Perez L, Cedazo-Minguez A, Pang ST, Norstedt G, Pousette A, Flores-Morales A
Oncogene 2010 Mar 11;29(10):1531-42
Oncogene 2010 Mar 11;29(10):1531-42
Ezrin is highly expressed in early thymocytes, but dispensable for T cell development in mice.
Shaffer MH, Huang Y, Corbo E, Wu GF, Velez M, Choi JK, Saotome I, Cannon JL, McClatchey AI, Sperling AI, Maltzman JS, Oliver PM, Bhandoola A, Laufer TM, Burkhardt JK
PloS one 2010 Aug 27;5(8):e12404
PloS one 2010 Aug 27;5(8):e12404
A signature predictive of disease outcome in breast carcinomas, identified by quantitative immunocytochemical assays.
Charpin C, Secq V, Giusiano S, Carpentier S, Andrac L, Lavaut MN, Allasia C, Bonnier P, Garcia S
International journal of cancer 2009 May 1;124(9):2124-34
International journal of cancer 2009 May 1;124(9):2124-34
Establishment and characterization of 4 new human pancreatic cancer cell lines: evidences of different tumor phenotypes.
Chifenti B, Morelli M, Zavaglia M, Coviello DA, Guerneri S, Santucci A, Paffetti A, Masetti M, Locci MT, Bertacca G, Capodanno A, Collecchi P, Campani D, Mosca F, Bevilacqua G, Cavazzana AO
Pancreas 2009 Mar;38(2):184-96
Pancreas 2009 Mar;38(2):184-96
Ezrin-radixin-moesin-binding phosphoprotein (EBP50), an estrogen-inducible scaffold protein, contributes to biliary epithelial cell proliferation.
Fouassier L, Rosenberg P, Mergey M, Saubaméa B, Clapéron A, Kinnman N, Chignard N, Jacobsson-Ekman G, Strandvik B, Rey C, Barbu V, Hultcrantz R, Housset C
The American journal of pathology 2009 Mar;174(3):869-80
The American journal of pathology 2009 Mar;174(3):869-80
Involvement of CD44v6 in InlB-dependent Listeria invasion.
Jung C, Matzke A, Niemann HH, Schwerk C, Tenenbaum T, Orian-Rousseau V
Molecular microbiology 2009 Jun;72(5):1196-207
Molecular microbiology 2009 Jun;72(5):1196-207
Aberrant epithelial morphology and persistent epidermal growth factor receptor signaling in a mouse model of renal carcinoma.
Morris ZS, McClatchey AI
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 16;106(24):9767-72
Proceedings of the National Academy of Sciences of the United States of America 2009 Jun 16;106(24):9767-72
Slit diaphragms contain tight junction proteins.
Fukasawa H, Bornheimer S, Kudlicka K, Farquhar MG
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1491-503
Journal of the American Society of Nephrology : JASN 2009 Jul;20(7):1491-503
Expression of ezrin is associated with invasion and dedifferentiation of hepatitis B related hepatocellular carcinoma.
Yeh CN, Pang ST, Chen TW, Wu RC, Weng WH, Chen MF
BMC cancer 2009 Jul 15;9:233
BMC cancer 2009 Jul 15;9:233
Ezrin silencing by small hairpin RNA reverses metastatic behaviors of human breast cancer cells.
Li Q, Wu M, Wang H, Xu G, Zhu T, Zhang Y, Liu P, Song A, Gang C, Han Z, Zhou J, Meng L, Lu Y, Wang S, Ma D
Cancer letters 2008 Mar 8;261(1):55-63
Cancer letters 2008 Mar 8;261(1):55-63
Nestin expression in repopulating mesangial cells promotes their proliferation.
Daniel C, Albrecht H, Lüdke A, Hugo C
Laboratory investigation; a journal of technical methods and pathology 2008 Apr;88(4):387-97
Laboratory investigation; a journal of technical methods and pathology 2008 Apr;88(4):387-97
Inhibition of T cell activation by cyclic adenosine 5'-monophosphate requires lipid raft targeting of protein kinase A type I by the A-kinase anchoring protein ezrin.
Ruppelt A, Mosenden R, Grönholm M, Aandahl EM, Tobin D, Carlson CR, Abrahamsen H, Herberg FW, Carpén O, Taskén K
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 15;179(8):5159-68
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 15;179(8):5159-68
Adhesion molecules as prognostic markers in pancreatic adenocarcinoma.
Torer N, Kayaselcuk F, Nursal TZ, Yildirim S, Tarim A, Nòyan T, Karakayali H
Journal of surgical oncology 2007 Oct 1;96(5):419-23
Journal of surgical oncology 2007 Oct 1;96(5):419-23
Ezrin and radixin both regulate the apical membrane localization of ABCC2 (MRP2) in human intestinal epithelial Caco-2 cells.
Yang Q, Onuki R, Nakai C, Sugiyama Y
Experimental cell research 2007 Oct 1;313(16):3517-25
Experimental cell research 2007 Oct 1;313(16):3517-25
A bipartite signal regulates the faithful delivery of apical domain marker podocalyxin/Gp135.
Yu CY, Chen JY, Lin YY, Shen KF, Lin WL, Chien CL, ter Beest MB, Jou TS
Molecular biology of the cell 2007 May;18(5):1710-22
Molecular biology of the cell 2007 May;18(5):1710-22
Association of ezrin expression in intestinal and diffuse gastric carcinoma with clinicopathological parameters and tumor type.
Bal N, Yildirim S, Nursal TZ, Bolat F, Kayaselcuk F
World journal of gastroenterology 2007 Jul 21;13(27):3726-9
World journal of gastroenterology 2007 Jul 21;13(27):3726-9
Ezrin in osteosarcoma: comparison between conventional high-grade and central low-grade osteosarcoma.
Park HR, Jung WW, Bacchini P, Bertoni F, Kim YW, Park YK
Pathology, research and practice 2006;202(7):509-15
Pathology, research and practice 2006;202(7):509-15
Androgen induction of prostate cancer cell invasion is mediated by ezrin.
Chuan YC, Pang ST, Cedazo-Minguez A, Norstedt G, Pousette A, Flores-Morales A
The Journal of biological chemistry 2006 Oct 6;281(40):29938-48
The Journal of biological chemistry 2006 Oct 6;281(40):29938-48
Differential tissue and subcellular expressionof ERM proteins in normal and malignant tissues: cytoplasmic ezrin expression has prognostic signficance for head and neck squamous cell carcinoma.
Madan R, Brandwein-Gensler M, Schlecht NF, Elias K, Gorbovitsky E, Belbin TJ, Mahmood R, Breining D, Qian H, Childs G, Locker J, Smith R, Haigentz M Jr, Gunn-Moore F, Prystowsky MB
Head & neck 2006 Nov;28(11):1018-27
Head & neck 2006 Nov;28(11):1018-27
Prognostic impact of immunohistochemical expression of ezrin in highly malignant soft tissue sarcomas.
Weng WH, Ahlén J, Aström K, Lui WO, Larsson C
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 1;11(17):6198-204
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Sep 1;11(17):6198-204
Ezrin expression in prostate cancer and benign prostatic tissue.
Valdman A, Fang X, Pang ST, Nilsson B, Ekman P, Egevad L
European urology 2005 Nov;48(5):852-7
European urology 2005 Nov;48(5):852-7
Stromal cells in hemangioblastoma: neuroectodermal differentiation and morphological similarities to ependymoma.
Ishizawa K, Komori T, Hirose T
Pathology international 2005 Jul;55(7):377-85
Pathology international 2005 Jul;55(7):377-85
Myocilin is expressed in the glomerulus of the kidney and induced in mesangioproliferative glomerulonephritis.
Goldwich A, Baulmann DC, Ohlmann A, Flügel-Koch C, Schöcklmann H, Tamm ER
Kidney international 2005 Jan;67(1):140-51
Kidney international 2005 Jan;67(1):140-51
Expression of ezrin in prostatic intraepithelial neoplasia.
Pang ST, Fang X, Valdman A, Norstedt G, Pousette A, Egevad L, Ekman P
Urology 2004 Mar;63(3):609-12
Urology 2004 Mar;63(3):609-12
Ezrin is essential for epithelial organization and villus morphogenesis in the developing intestine.
Saotome I, Curto M, McClatchey AI
Developmental cell 2004 Jun;6(6):855-64
Developmental cell 2004 Jun;6(6):855-64
Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients.
Moilanen J, Lassus H, Leminen A, Vaheri A, Bützow R, Carpén O
Gynecologic oncology 2003 Aug;90(2):273-81
Gynecologic oncology 2003 Aug;90(2):273-81
Gene expression profiling of androgen deficiency predicts a pathway of prostate apoptosis that involves genes related to oxidative stress.
Pang ST, Dillner K, Wu X, Pousette A, Norstedt G, Flores-Morales A
Endocrinology 2002 Dec;143(12):4897-906
Endocrinology 2002 Dec;143(12):4897-906
Thyroid hormone-deficient period prior to the onset of hearing is associated with reduced levels of beta-tectorin protein in the tectorial membrane: implication for hearing loss.
Knipper M, Richardson G, Mack A, Müller M, Goodyear R, Limberger A, Rohbock K, Köpschall I, Zenner HP, Zimmermann U
The Journal of biological chemistry 2001 Oct 19;276(42):39046-52
The Journal of biological chemistry 2001 Oct 19;276(42):39046-52
ERM-dependent movement of CD43 defines a novel protein complex distal to the immunological synapse.
Allenspach EJ, Cullinan P, Tong J, Tang Q, Tesciuba AG, Cannon JL, Takahashi SM, Morgan R, Burkhardt JK, Sperling AI
Immunity 2001 Nov;15(5):739-50
Immunity 2001 Nov;15(5):739-50
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Ezrin in U2OS cells. Cells fixed with 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 (Product # 28314) in PBS for 15 minutes at room temperature and blocked with 2% BSA in PBST (Product # 37525) for 30 minutes at room temperature. Cells were treated with Peroxidase Suppressor (Product # 35000), probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an HRP-conjugated goat anti-mouse IgG (H+L) secondary antibody (Product # 31431) at a dilution of 1:1000 for 30 minutes at room temperature. Chromogenic detection was performed using Metal Enhanced DAB Substrate Kit (Product # 34065). Images were taken on a Zeiss Axio Observer microscope at 20X magnification (x1.6 Optovar ~ 32X).
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Ezrin in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 (Product # 28314) in TBST (Product # 28376) for 30 minutes at room temperature and blocked with Universal Blocker (from Product # 03-6000) for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBST, and incubated with an alkaline phosphatase-conjugated goat anti-mouse IgG (H+L) secondary antibody (Product # 31320) at a dilution of 1:250 for 30 minutes at room temperature. Chromogenic detection was performed using 1-Step NBT/BCIP (Product # 34042). Images were taken on a Zeiss Axio Observer microscope at 20X magnification (x1.6 Optovar ~ 32X).
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phalloidin (orange) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (Product # 21835) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phalloidin (magenta) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 633 Phalloidin (Product # 21840) at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Phalloidin (dark red) and Ezrin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Product # 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 680 Phalloidin (Product # 21839) at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst (Product # 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Zeiss Axio Observer Z1 microscope at 20X magnification.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human breast cancer tissue sections. To expose target proteins, heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC. Following antigen retrieval, tissues were blocked in 3% BSA (Product # 37525) in PBST for 30 minutes at room temperature and then probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for 1 hour in a humidified chamber. Tissues were washed extensively with PBS/0.025% Tween-20 (Product # 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000) for 30 minutes at room temperature. Detection was performed using an HRP-conjugated goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:500 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit (Product # 34065). Images were taken on a Zeiss Axiovision microscope at 40X magnification.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry was performed on paraffin-embedded human colon cancer tissue sections. To expose target proteins, heat-induced epitope retrieval was performed using 10mM sodium citrate (pH 6.0) buffer for 20 minutes at 95ºC. Following antigen retrieval, tissues were blocked in 3% BSA (Product # 37525) in PBST for 30 minutes at room temperature and then probed with an Ezrin monoclonal antibody (Product # MA5-13862) at a dilution of 1:100 for 1 hour in a humidified chamber. Tissues were washed extensively with PBS/0.025% Tween-20 (Product # 28314) and endogenous peroxidase activity quenched with Peroxidase Suppressor (Product # 35000) for 30 minutes at room temperature. Detection was performed using an HRP-conjugated goat anti-mouse IgG-HRP secondary antibody (Product # 31430) at a dilution of 1:500 followed by colorimetric detection using Metal Enhanced DAB Substrate Kit (Product # 34065). Images were taken on a Zeiss Axiovision microscope at 40X magnification.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human lung stained with Ezrin antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of bronchio-epithelial cells.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Ezrin using Ezrin Monoclonal Antibody (Product # MA5-13859) on Native Human Raji Cells.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Ezrin using Ezrin Monoclonal Antibody (Product # MA5-13859) on denatured Human Raji Cells.
- Conjugate
- Biotin