Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28220 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFS1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, mouse brain, rat brain. Predicted reactivity: Mouse (91%), Rat (92%), Xenopus laevis (82%), Chicken (86%), Chimpanzee (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references A novel voluntary weightlifting model in mice promotes muscle adaptation and insulin sensitivity with simultaneous enhancement of autophagy and mTOR pathway.
Cui D, Drake JC, Wilson RJ, Shute RJ, Lewellen B, Zhang M, Zhao H, Sabik OL, Onengut S, Berr SS, Rich SS, Farber CR, Yan Z
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jun;34(6):7330-7344
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2020 Jun;34(6):7330-7344
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFS1 Polyclonal Antibody (Product # PA5-28220). Sample (30 µg of whole cell lysate). A: HepG2. 7.5% SDS PAGE. NDUFS1 Polyclonal Antibody (Product # PA5-28220) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFS1 Polyclonal Antibody detects NDUFS1 protein by western blot analysis. A. 50 µg rat brain lysate/extract.7.5% SDS-PAGE. NDUFS1 Polyclonal Antibody (Product # PA5-28220) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NDUFS1 Polyclonal Antibody (Product # PA5-28220). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 7.5% SDS PAGE. NDUFS1 Polyclonal Antibody (Product # PA5-28220) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NDUFS1 Polyclonal Antibody detects NDUFS1 protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded ovarian carcinoma. NDUFS1 Polyclonal Antibody (Product # PA5-28220) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.