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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- 17464-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FASTKD2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ILLDECSKV VLDNIHGCPL RIMINILQSC KDLQYHNLDL FKGLADYVAA TFDIWKFRKV LFILILFENL GFRPVGLMDL FMKRIVEDPE SLNMKNILSI LHTYSSLNHV YKCQNKEQFV EVMASALTGY LHTISSENLL DAVYSFCLMN YFPLAPFNQL LQKDIISELL TSDDMKNAYK LHTLDTCLKL DDTVYLRDIA LSLPQLPREL PSSHTNAKVA EVLSSLLGGE GHFSKDVHLP HNYHIDFEIR MDTNRNQVLP LSDVDTTSAT DIQRVAVLCV SRSAYCLGSS HPRGFLAMKM RHLNAMGFHV ILVNNWEMDK LEMEDAVTFL KTKIYSVEAL PVAAVNVQST Q (361-710 aa encoded by BC001544)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.41 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of anti-FASTKD2 (17464-1-AP) with overexpressed protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse heart tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 17464-1-AP (FASTKD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti- FASTKD2 (17464-1-AP) with FASTKD2-YFP overexpression in COS7 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-FASTKD2 (17464-1-AP) with U2OS cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IF result of anti-FASTKD2 (17464-1-AP) with COS7 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-FASTKD2 (IP:17464-1-AP, 4ug; Detection:17464-1-AP 1:300) with mouse heart tissue lysate 4000ug.
