Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Other assay [4]
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- Product number
- PA5-101340 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLF7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Antibody detects endogenous levels of total KLF7.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Krüppel-like factor 7 attenuates hippocampal neuronal injury after traumatic brain injury.
Li WY, Fu XM, Wang ZD, Li ZG, Ma D, Sun P, Liu GB, Zhu XF, Wang Y
Neural regeneration research 2022 Mar;17(3):661-672
Neural regeneration research 2022 Mar;17(3):661-672
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KLF7 in COLO205 cells. Samples were incubated with KLF7 polyclonal antibody (Product # PA5-101340).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KLF7 in COLO205 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with KLF7 polyclonal antibody (Product # PA5-101340) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of KLF7 in COLO205 cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with KLF7 polyclonal antibody (Product # PA5-101340) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 Efficacy of AAV-KLF7 gene transfer to HT22 cells following stretch and OGD in vitro . (A) Representative western blot analysis of KLF7 expression in cultured HT22 cells at 1 day after stretch and OGD in vitro . (B) Quantitative results of the relative expression of KLF7 protein detected by western blot. The relative expression is expressed as the optical density ratio to GAPDH. (C) qRT-PCR analysis of KLF7 mRNA expression in the cultured HT22 cells at 1 day after stretch and OGD. (D) Immunofluorescence staining of KLF7 (green, stained by fluorescein isothiocyanate) and betaIII-tubulin (red, stained by Alexa 555) with nuclear staining (DAPI; blue) in the three groups. The merged image shows that HT22 cells were co-labeled by KLF7, betaIII-tubulin, and DAPI. The expression of KLF7 was increased after AAV-KLF7 infection compared with the other groups, and beta-III-tubulin expression was also elevated in AAV-KLF7 cells compared with AAV-NC cells. Scale bars: 100 mum. (E, F) Quantitative results of the immunopositivity of KLF7 (E) and betaIII-tubulin (F). Data are expressed as the mean +- SD. Each experiment was repeated three (A-C) or six (D-F) times. * P < 0.05, ** P < 0.01, *** P < 0.001 (one-way analysis of variance followed by Tukey's post hoc test). AAV: Adeno-associated virus; DAPI: 4',6-diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; KLF7: Kruppel-like factor 7; NC: negative control; OGD: oxygen-glucose deprivation; qRT-PCR: quantitative
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 4 Involvement of the JAK2/STAT3 signaling pathway in the neuroprotective effects of KLF7. (A) Representative western blot of p-JAK2, t-JAK2, p-STAT3, and t-STAT3 expression in cultured HT22 cells at 1 day after stretch and OGD treatment. (B, C) Quantitative results of the relative optical densities of p-JAK2/t-JAK2 and p-STAT3/t-STAT3. (D) Co-immunoprecipitation analysis of the interactions between KLF7 and p-STAT3 in stretch- and OGD-damaged HT22 cells at 1 day, revealing a physical interaction between KLF7 and p-STAT3. Data are expressed as the mean +- SD. Each experiment was repeated three times. * P < 0.05, vs . control group; # P < 0.05, vs . stretch + OGD + AAV-NC group; & P < 0.05, vs . stretch + OGD + AAV-KLF7 group (one-way analysis of variance followed by Tukey's post hoc test). AAV: Adeno-associated virus; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; IB: immunoblotting; IP: immunoprecipitation; KLF7: Kruppel-like factor 7; NC: negative control; OGD: oxygen-glucose deprivation; p-JAK2: phospho-Janus kinase 2; t-JAK2: total-Janus kinase 2; p-STAT3: phospho-signal transducer and activator of transcription 3; t-STAT3: total-signal transducer and activator of transcription 3.
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- Invitrogen Antibodies (provider)
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- Experimental details
- Figure 6 Changes in KLF7 in the mouse hippocampus following TBI. (A) Western blot analysis showing the protein expression of KLF7 in the hippocampus after TBI. (B) Quantitative results of KLF7 protein expression, displayed as relative optical density normalized to GAPDH. (C) Quantitative results of KLF7 mRNA at different times by qRT-PCR. Data are expressed as the mean +- SD ( n = 3). *** P < 0.001 (one-way analysis of variance followed by Tukey's post hoc test). (D) To explore the expression of KLF7 in different cell types, double-labeling immunohistochemistry was performed for KLF7 (purple, stained by Cy3), GFAP (red, stained by Alexa 555), NeuN (green, stained by fluorescein isothiocyanate), and DAPI (blue). KLF7 was expressed in cortical and hippocampal neurons of mice 3 days after TBI. The arrow indicates KLF7 expression in a NeuN-positive cell. No GFAP-positive cells were observed to express KLF7. Scale bars: 200 mum. DAPI: 4',6-Diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GFAP: glial fibrillary acidic protein; KLF7: Kruppel-like factor 7; NS: not significant; qRT-PCR: quantitative real-time polymerase chain reaction; TBI: traumatic brain injury.
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- Experimental details
- Figure 8 KLF7 protects hippocampal neurons against TBI-induced apoptosis at 3 days after surgery. (A) Representative western blots of KLF7, Bcl-2, Bax, and C-caspase3 expression in the ipsilateral hippocampus at 3 days after TBI. (B-E) Quantitative results of the expression of KLF7 (B), Bcl-2 (C), Bax (D), and C-caspase3 (E). TBI decreased the expression of Bcl-2 and increased that of Bax and C-caspase3 compared with the sham group. Moreover, the expression of Bcl-2 was elevated in the TBI + AAV-KLF7 group compared with the TBI + AAV-NC group, while the expression of Bax and C-caspase3 was decreased. (F) Apoptotic cells in the ipsilateral hippocampus were stained by TUNEL (red) with nuclear staining (DAPI; blue). TBI increased the number of TUNEL-positive cells compared with the sham group, and the number of TUNEL-positive cells in the TBI + AAV-KLF7 group was lower than that in the TBI + AAV-NC group. The merged image shows that cells were double-labeled with TUNEL and DAPI. Scale bars: 100 mum. (G) Quantitative results of the TUNEL-positive cells in the ipsilateral hippocampus. Data are expressed as the mean +- SD ( n = 3 in A-E, n = 6 in F-G). * P < 0.05, *** P < 0.001 (one-way analysis of variance followed by Tukey's post hoc test). AAV: Adeno-associated virus; Bax: Bcl-2-associated X protein; Bcl-2: B-cell lymphoma 2; C-caspase3: cleaved caspase-3; DAPI: 4',6-diamidino-2-phenylindole; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; KLF7: Kruppel-like factor 7; NC: negat