Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 686302 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#686302, RRID:AB_2616882
- Product name
- Purified anti-FOXO3
- Antibody type
- Monoclonal
- Antigen
- Human FOXO3 recombinant protein (424-673 a.a.) expressed in E. coli.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- W15111A
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from human SY5Y cells (lane 1), RPMI8226 cells (lane 2), HeLa cells (lane 3), and mouse NIH-3T3 cells (lane 4) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-FOXO3 (clone W15111A) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG Antibody and chemiluminescence detection.
- Conjugate
- Unconjugated