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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- ARG55374 - Provider product page
- Provider
- Arigo
- Product name
- anti-IDH1 antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 116-143 (Center) of Human IDH1.
- Description
- This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HepG2 cell line and mouse liver tissue lysates stained with ARG55374 anti-IDH1 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of HepG2, MCF-7 cell line, human liver and rat liver tissue lysate(from left to right) stained with ARG55374 anti-IDH1 antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HepG2 cell stained with ARG55374 anti-IDH1 antibody (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: formalin-fixed and paraffin-embedded human hepatocarcinoma tissue stained with ARG55374 anti-IDH1 antibody.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. prostate section stained with ARG55374 anti-IDH1 antibody at 1:100 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: 293 cells stained with ARG55374 anti-IDH1 antibody (right histogram) or without primary antibody control (left histogram), followed by incubation with FITC labelled secondary antibody.
