F50421

antibody from NSJ Bioreagents

Targeting: IDH1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F50421

Provider

NSJ Bioreagents

Product name

IDH Antibody

Antibody type

Polyclonal

Antigen

A portion of amino acids 63-90 from the human protein was used as the immunogen for this IDH antibody.

Description

Purified antibody

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

80 µl, 400 µl

Storage

Aliquot the IDH antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot analysis of IDH antibody and mouse liver tissue and 293 lysate. Predicted molecular weight ~46 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of IDH antibody with HepG2 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI nuclear counterstain (blue).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC analysis of FFPE human brain stained with IDH antibody

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometric analysis of 293 cells using IDH antibody (bottom histogram) compared to a negative control (top histogram).