Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-15560 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PTEN Monoclonal Antibody (1B8)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15560 targets PTEN in indirect ELISA, ICC, IF, FACS, and WB applications and shows reactivity with Human and mouse samples. The MA5-15560 immunogen is purified recombinant fragment of PTEN expressed in E. Coli. MA5-15560 detects PTEN which has a predicted molecular weight of approximately 54kDa.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1B8
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTEN using PTEN monoclonal antibody (Product # MA5-15560) in HeLa (1) and NIH/3T3 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PTEN using PTEN monoclonal antibody (Product # MA5-15560) in HeLa (1) and NIH/3T3 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa (left) and HepG2 (right) cells using PTEN monoclonal antibody (Product # MA5-15560) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with DY-554 phalloidin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using PTEN monoclonal antibody (Product # MA5-15560) (right) and negative control (left).