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LearnH00200576-M01
antibody from Novus Biologicals
Targeting: PIKFYVE
FAB1, KIAA0981, MGC40423, p235, PIP5K, PIP5K3, ZFYVE29
Western blot ELISA Immunocytochemistry
Immunoprecipitation Immunohistochemistry Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00200576-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00200576-M01, RRID:AB_547056
- Product name
- Mouse Monoclonal PIKFyve/PIP5K3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PIP5K3 - phosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type III
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages.
Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine.
PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death.
Morioka S, Nigorikawa K, Hazeki K, Ohmura M, Sakamoto H, Matsumura T, Takasuga S, Hazeki O
Experimental cell research 2017 Aug 15;357(2):252-259
Experimental cell research 2017 Aug 15;357(2):252-259
Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine.
Takasuga S, Horie Y, Sasaki J, Sun-Wada GH, Kawamura N, Iizuka R, Mizuno K, Eguchi S, Kofuji S, Kimura H, Yamazaki M, Horie C, Odanaga E, Sato Y, Chida S, Kontani K, Harada A, Katada T, Suzuki A, Wada Y, Ohnishi H, Sasaki T
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 29;110(5):1726-31
Proceedings of the National Academy of Sciences of the United States of America 2013 Jan 29;110(5):1726-31
PIKfyve regulates CaV1.2 degradation and prevents excitotoxic cell death.
Tsuruta F, Green EM, Rousset M, Dolmetsch RE
The Journal of cell biology 2009 Oct 19;187(2):279-94
The Journal of cell biology 2009 Oct 19;187(2):279-94
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi ( Cat # H00200576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody (M01), clone 6C7 (Cat # H00200576-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody (M01), clone 6C7.Lane 1: PIP5K3 transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Detection limit for recombinant GST tagged PIP5K3 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PIKFyve/PIP5K3 Antibody (6C7) [H00200576-M01] - Analysis of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.
