PA5-118164

antibody from Invitrogen Antibodies

Targeting: PIKFYVE FAB1, KIAA0981, MGC40423, p235, PIP5K, PIP5K3, ZFYVE29

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-118164

Provider

Invitrogen Antibodies

Product name

PIKFYVE Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PIKFYVE Polyclonal Antibody (Product # PA5-118164) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 237 kDa. Observed band size: 237 kDa. (We are unsure of the identity of these extra bands).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PIKFYVE Polyclonal Antibody (Product # PA5-118164) at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 237 kDa. Observed band size: 237 kDa. (We are unsure of the identity of these extra bands).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human PIKFYVE in human kidney with PIKFYVE Polyclonal Antibody (Product # PA5-118164, 1:300 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

PIKFYVE Immunoprecipitation using: Lane A: 0.5 mg Jurkat Whole Cell Lysate 4 µL with PIKFYVE Polyclonal Antibody (Product # PA5-118164) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: PIKFYVE Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 237 kDa. Observed band size: 237 kDa.