PA5-119883

antibody from Invitrogen Antibodies

Targeting: RPE

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-119883

Provider

Invitrogen Antibodies

Product name

Ribulose-phosphate 3-epimerase Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Mouse heart tissue lysate, human heart tissue lysate, human small intestine tissue, SH-SY5Y.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RPE on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Ribulose-phosphate 3-epimerase Polyclonal Antibody (Product # PA5-119883) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Mouse heart tissue lysate. Lane 2: Human heart tissue lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of of RPE in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with Ribulose-phosphate 3-epimerase Polyclonal Antibody (Product # PA5-119883) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of of RPE in SH-SY5Y cells (green). Methanol fixed cells were blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with Ribulose-phosphate 3-epimerase Polyclonal Antibody (Product # PA5-119883) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human small intestine tissue using Ribulose-phosphate 3-epimerase Polyclonal Antibody (Product # PA5-119883). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the Ribulose-phosphate 3-epimerase antibody at a dilution of 1:400 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.